Evidence map›Paper›PMID 28324392›Full record

Article3 Biotech2015

Validation of dbEST-SSRs and transferability of some other solanaceous species SSR in ashwagandha [Withania Somnifera (L.) Dunal].

Eva K Parmar, Ranbir S Fougat, Chandni B Patel, Harshvardhan N Zala, Mahesh A Patel, Swati K Patel, Sushil Kumar

Open access · hybridAbstract read
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In one paragraph

Article in 3 Biotech, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
1.4field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 8 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Eva K ParmarDepartment of Agricultural Biotechnology, Anand Agricultural University, Anand, Gujarat, 388 110, India.
Ranbir S FougatDepartment of Agricultural Biotechnology, Anand Agricultural University, Anand, Gujarat, 388 110, India.
Chandni B PatelDepartment of Agricultural Biotechnology, Anand Agricultural University, Anand, Gujarat, 388 110, India.
Harshvardhan N ZalaDepartment of Agricultural Biotechnology, Anand Agricultural University, Anand, Gujarat, 388 110, India.
Mahesh A PatelDepartment of Agricultural Biotechnology, Anand Agricultural University, Anand, Gujarat, 388 110, India.
Swati K PatelMedicinal and Aromatic Plants Unit, Anand Agricultural University, Anand, Gujarat, 388 110, India.
Sushil KumarDepartment of Agricultural Biotechnology, Anand Agricultural University, Anand, Gujarat, 388 110, India. sushil254386@yahoo.com.
Anand Agricultural University · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cross-species transferability and expressed sequence tags (ESTs) in public databases are cost-effective means for developing simple sequence repeats (SSRs) for less-studied species like medicinal plants. In this study, 11 EST-SSR markers developed from 742 available ESTs of Withania Somnifera EST sequences and 95 SSR primer pairs derived from other solanaceous crops (tomato, eggplant, chili, and tobacco) were utilized for their amplification and validation. Out of 11, 10 EST-SSRs showed good amplification quality and produced 13 loci with a product size ranging between 167 and 291 bp. Similarly, of the 95 cross-genera SSR loci assayed, 20 (21 %) markers showed the transferability of 5, 27, 32, and 14.2 % from eggplant, chili, tomato, and tobacco, respectively, to ashwagandha. In toto, these 30 SSR markers reported here will be valuable resources and may be applicable for the analysis of intra- and inter-specific genetic diversity in ashwagandha for which till date no information about SSR is available.

Indexed as

AshwagandhaCross-genera transferabilityExpressed sequence tagsGenomic SSRMedicinal plantWithania somnifera

Identifiers

PMID28324392
PMCPMC4624151
OpenAlexW2044161020

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.