Article3 Biotech2014
Specific oligonucleotide primers for detection of endoglucanase positive Bacillus subtilis by PCR.
Article in 3 Biotech, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed.
- Comparative Genomic, Metabolic and Transcriptomic Profiling of Bacterial Isolates Reveals Strain-Specific Adaptation and Safety Traits.International journal of molecular sciences · 2026Article
- Knockouts of Sulfur Metabolism Genes Induce Chronic Inflammation and Immune Dysregulation inAntioxidants (Basel, Switzerland) · 2026Article
- Dose-dependent effects of an indigenousOpen veterinary journal · 2026Article
- Bio-Priming withJournal of fungi (Basel, Switzerland) · 2024Article
- Chromosomal barcodes for simultaneous tracking of near-isogenic bacterial strains in plant microbiota.Nature microbiology · 2024Article
- Characterization of a Plant Growth-Promoting EndohyphalPolish journal of microbiology · 2023Article
- DNA-Immobilized Fluorescent Polystyrene Nanoparticles as Probes with Tunable Detection Limits.ACS omega · 2022Article
- Highly Efficient Biotransformation and Production of Selenium Nanoparticles and Polysaccharides Using Potential Probiotic Bacillus subtilis T5Metabolites · 2022Article
- Discrimination ofJournal of microbiology and biotechnology · 2022Article
- Study on the Identification Methods for Effective Microorganisms in Commercially Available Organic Agriculture Materials.Microorganisms · 2020Article
- Understanding the ontogeny and succession of Bacillus velezensis and B. subtilis subsp. subtilis by focusing on kimchi fermentation.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A polymerase chain reaction (PCR) assay was developed for discrimination of Bacillus subtilis from other members of B. subtilis group as well as rapid identification from environmental samples. Primers ENIF and EN1R from endoglucanase gene were used to amplify a1311 bp DNA fragment. The specificity of the primers was tested with seven reference strains and 28 locally isolated strains of endoglucanase positive Bacillus species. The PCR product was only produced from B. subtilis. The results demonstrated high specificity of two oligonucleotides for B. subtilis. This species-specific PCR method provides a quick, simple, powerful and reliable alternative to conventional methods in the detection and identification of B. subtilis. To our knowledge this is the first report of a B. subtilis specific primer set.
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Registered trials
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