Evidence map›Paper›PMID 28324307›Full record

Article3 Biotech2014

Statistical media optimization and cellulase production from marine Bacillus VITRKHB.

Kunal Singh, Kumari Richa, Himadri Bose, Loganathan Karthik, Gaurav Kumar, Kokati Venkata Bhaskara Rao

Abstract read
In one paragraph

Article in 3 Biotech, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Isolation and enzyme bioprospection of bacteria associated toBiotechnology reports (Amsterdam, Netherlands) · 2021
    Article
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Kunal SinghMolecular and Microbiology Research Laboratory, Environmental Biotechnology Division, School of Bio Sciences and Technology, VIT University, Vellore, 632014, Tamil Nadu, India.
Kumari RichaMolecular and Microbiology Research Laboratory, Environmental Biotechnology Division, School of Bio Sciences and Technology, VIT University, Vellore, 632014, Tamil Nadu, India.
Himadri BoseMolecular and Microbiology Research Laboratory, Environmental Biotechnology Division, School of Bio Sciences and Technology, VIT University, Vellore, 632014, Tamil Nadu, India.
Loganathan KarthikMolecular and Microbiology Research Laboratory, Environmental Biotechnology Division, School of Bio Sciences and Technology, VIT University, Vellore, 632014, Tamil Nadu, India.
Gaurav KumarMolecular and Microbiology Research Laboratory, Environmental Biotechnology Division, School of Bio Sciences and Technology, VIT University, Vellore, 632014, Tamil Nadu, India.
Kokati Venkata Bhaskara RaoMolecular and Microbiology Research Laboratory, Environmental Biotechnology Division, School of Bio Sciences and Technology, VIT University, Vellore, 632014, Tamil Nadu, India. kokatibhaskar@yahoo.co.in.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Marine Bacillus species are potent producers of novel enzymes. Marine Bacillus VITRKHB was observed to be efficient for cellulolytic activity. It was employed for the production of extracellular cellulase. Cellulase was partially purified to 1.6-fold in a stepwise manner by ammonium sulfate precipitation, dialysis, and DEAE ion exchange chromatography. The molecular weight of purified protein was found to be about 33 kDa by SDS-PAGE. Its specific activity was recorded as 1.92 IU/mg. The effect of various carbon and nitrogen sources on cellulase production was investigated. The maximum enzyme activity was recorded in the fermentation media containing xylose as carbon source and beef extract as nitrogen source. The combined interactive effect of different variables on cellulase production was studied by response surface methodology. The optimized combination of variables for maximum enzyme activity was determined as; xylose 5.0 %, beef extract 6.9 %, pH 7.83, NaCl 1.17, and temperature 25.84 °C, after 24 h of incubation.

Indexed as

Bacillus VITRKHBCellulaseRSMXyloseYeast extract

Identifiers

PMID28324307
PMCPMC4235885

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.