ArticleEndocrinology2017
Transcriptional Dynamics of Cultured Human Villous Cytotrophoblasts.
Article in Endocrinology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 24 papers, 3 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 3 syntheses or guidelines pooled it, 31 citations in OpenAlex.
- Revealing the molecular landscape of human placenta: a systematic review and meta-analysis of single-cell RNA sequencing studies.Human reproduction update · 2024Pooled it
- Integrated analysis of transcriptomic datasets to identify placental biomarkers of spontaneous preterm birth.Placenta · 2022Pooled it
- Current approaches and developments in transcript profiling of the human placenta.Human reproduction update · 2020Pooled it
- Profiling the cytotoxic effects of naled and other pesticides in primary human placental cytotrophoblasts.Toxicological sciences : an official journal of the Society of Toxicology · 2025Article
- NOTUM-mediated WNT silencing drives extravillous trophoblast cell lineage development.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- NOTUM-MEDIATED WNT SILENCING DRIVES EXTRAVILLOUS TROPHOBLAST CELL LINEAGE DEVELOPMENT.bioRxiv : the preprint server for biology · 2024Article
- Evaluation of novel biomarkers for early pregnancy outcome prediction†.Biology of reproduction · 2024Article
- Perfluorooctanoic acid induces transcriptomic alterations in second trimester human cytotrophoblasts.Toxicological sciences : an official journal of the Society of Toxicology · 2023Article
- Proteomic analyses of primary human villous trophoblasts exposed to flame retardant BDE-47 using SWATH-MS.Toxicology · 2023Article
- CITED2 is a conserved regulator of the uterine-placental interface.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- The multifaceted role of GCM1 during trophoblast differentiation in the human placenta.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Functional reorganization of monoamine transport systems during villous trophoblast differentiation: evidence of distinct differences between primary human trophoblasts and BeWo cells.Reproductive biology and endocrinology : RB&E · 2022Article
- NRF2 Serves a Critical Role in Regulation of Immune Checkpoint Proteins (ICPs) During Trophoblast Differentiation.Endocrinology · 2022Article
- The Placenta's Role in Sexually Dimorphic Fetal Growth Strategies.Reproductive sciences (Thousand Oaks, Calif.) · 2022Review
- Primary Trophoblast Cultures: Characterization of HLA Profiles and Immune Cell Interactions.Frontiers in immunology · 2022Article
- A Role for Gene-Environment Interactions in Autism Spectrum Disorder Is Supported by Variants in Genes Regulating the Effects of Exposure to Xenobiotics.Frontiers in neuroscience · 2022Article
- Global proteomic analyses of human cytotrophoblast differentiation/invasion.Development (Cambridge, England) · 2021Article
- Differences in cytochrome p450 enzyme expression and activity in fetal and adult tissues.Placenta · 2020Article
- Cytotrophoblast extracellular vesicles enhance decidual cell secretion of immune modulators via TNFα.Development (Cambridge, England) · 2020Article
- Use of a human embryonic stem cell model to discover GABRP, WFDC2, VTCN1 and ACTC1 as markers of early first trimester human trophoblast.Molecular human reproduction · 2020Article
Corrections and comments
- Erratum issued
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
During human pregnancy, cytotrophoblasts (CTBs) play key roles in uterine invasion, vascular remodeling, and anchoring of the feto-placental unit. Due to the challenges associated with studying human placentation in utero, cultured primary villous CTBs are used as a model of the differentiation pathway that leads to invasion of the uterine wall. In vitro, CTBs emulate in vivo cell behaviors, such as migration, aggregation, and substrate penetration. Although some of the molecular features related to these cell behaviors have been described, the underlying mechanisms, at a global level, remain undefined at midgestation. Thus, in this study, we characterized second-trimester CTB differentiation/invasion in vitro, correlating the major morphological transitions with the transcriptional changes that occurred at these steps. After plating on Matrigel as individual cells, CTBs migrated toward each other and formed multicellular aggregates. In parallel, using a microarray approach, we observed differentially expressed (DE) genes across time, which were enriched for numerous functions, including cell migration, vascular remodeling, morphogenesis, cell communication, and inflammatory signaling. DE genes encoded several molecules that we and others previously linked to critical CTB function in vivo, suggesting that the novel DE molecules we discovered played important roles. Immunolocalization confirmed that CTBs in situ gave a signal for two of the most highly expressed genes in vitro. In summary, we characterized, at a global level, the temporal dynamics of primary human CTB gene expression in culture. These data will enable future analyses of various types of in vitro perturbations-for example, modeling disease processes and environmental exposures.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.