ArticlePloS one2017
Establishment of transient gene expression systems in protoplasts from Liriodendron hybrid mesophyll cells.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24 citing papers in PubMed, 48 citations in OpenAlex.
- Systematic Evaluation of Mesophyll Protoplast Isolation and PEG-Mediated Transient Expression inPlants (Basel, Switzerland) · 2026Article
- Genomic survey and expression analysis of the CDPK genes in Liriodendron Chinense to explore their potential functions under multiple abiotic stresses.BMC genomics · 2026Article
- Establishing a reliable protoplast system for grapevine: isolation, transformation, and callus induction.Protoplasma · 2025Article
- The Role ofPlants (Basel, Switzerland) · 2024Article
- Genomic survey and expression analysis of LcARFs reveal multiple functions to somatic embryogenesis in Liriodendron.BMC plant biology · 2024Article
- Establishment of a glucocorticoid inducible system for regulating somatic embryogenesis inForestry research · 2024Article
- Advances inFrontiers in plant science · 2024Article
- Isolation, Purification, and Application of Protoplasts and Transient Expression Systems in Plants.International journal of molecular sciences · 2023Review
- Article
- AT-Hook Transcription Factors Show Functions inPlants (Basel, Switzerland) · 2023Article
- Optimization of Isolation and Transformation of Protoplasts fromInternational journal of molecular sciences · 2023Article
- Protoplast isolation and transient transformation system forFrontiers in plant science · 2023Article
- Comprehensive transcriptomic analysis of age-, dark-, and salt-induced senescence reveals underlying mechanisms and key regulators of leaf senescence inFrontiers in plant science · 2023Article
- Genome-wide identification of the Liriodendron chinense WRKY gene family and its diverse roles in response to multiple abiotic stress.BMC plant biology · 2022Article
- Article
- Optimization of Protoplast Isolation from Leaf Mesophylls of Chinese Cabbage (Plants (Basel, Switzerland) · 2021Article
- Physiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
- The role of γ-aminobutyric acid in aluminum stress tolerance in a woody plant, Liriodendron chinense × tulipifera.Horticulture research · 2021Article
- Selection of reference genes for gene expression analysis in Liriodendron hybrids' somatic embryogenesis and germinative tissues.Scientific reports · 2021Article
- Highly Efficient Leaf Base Protoplast Isolation and Transient Expression Systems for Orchids and Other Important Monocot Crops.Frontiers in plant science · 2021Article
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Liriodendron is a genus of the magnolia family comprised of two flowering tree species that produce hardwoods of great ecological and economic value. However, only a limited amount of genetic research has been performed on the Liriodendron genus partly because transient or stable transgenic trees have been difficult to produce. In general, transient expression systems are indispensable for rapid, high-throughput screening and systematic characterization of gene functions at a low cost; therefore, development of such a system for Liriodendron would provide a necessary step forward for research on Magnoliaceae and other woody trees. Herein, we describe an efficient and rapid protocol for preparing protoplasts from the leaf mesophyll tissue of a Liriodendron hybrid and an optimized system for polyethylene glycol-mediated transient transfection of the protoplasts. Because the leaves of the Liriodendron hybrid are waxy, we formulated an enzyme mix containing 1.5% (w/v) Cellulase R-10, 0.5% (w/v) Macerozyme R-10, and 0.1% (w/v) Pectolyase Y-23 to efficiently isolate protoplasts from the Liriodendron hybrid leaf mesophyll tissue in 3 h. We optimized Liriodendron protoplast transfection efficiency by including 20 μg plasmid DNA per 104 protoplasts, a transformation time of 20 min, and inclusion of 20% (w/v) polyethylene glycol 4000. After integrating the Liriodendron WOX1 gene into pJIT166-GFP to produce a WOX1-GFP fusion product and transfecting it into isolated protoplasts, LhWOX1-GFP was found to localize to the nucleus according to its green fluorescence.
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