ArticlePloS one2017
Selection and validation of reference genes for qRT-PCR analysis during biological invasions: The thermal adaptability of Bemisia tabaci MED.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Real time PCR identification ofOpen veterinary journal · 2026Article
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- Selection and validation of reference genes for RT-qPCR-based analyses ofFrontiers in physiology · 2022Article
- Characterization of Chromatin Remodeling Genes Involved in Thermal Tolerance of Biologically InvasiveFrontiers in physiology · 2022Article
- Selection and Validation of Reference Genes for Quantitative Real-Time PCR Normalization inFrontiers in physiology · 2022Article
- The Role of Cytochrome P450 4C1 and Carbonic Anhydrase 3 in Response to Temperature Stress inInsects · 2021Article
- Reference Gene Selection for Normalizing Gene Expression inFrontiers in physiology · 2021Article
- Selection and Validation of Reference Genes for RT-qPCR Normalization inFrontiers in physiology · 2021Article
- Comprehensive Assessment of Candidate Reference Genes for Gene Expression Studies Using RT-qPCR inGenes · 2020Article
- Selection of reference genes for normalization of RT-qPCR data in gene expression studies in Anthonomus eugenii Cano (Coleoptera: Curculionidae).Scientific reports · 2020Article
- Using RNA Interference to Reveal the Function of Chromatin Remodeling Factor ISWI in Temperature Tolerance inInsects · 2020Article
- Identification and Validation of Reference Genes for Quantitative Gene Expression Analysis inFrontiers in physiology · 2020Article
- Selection of Reference Genes for Normalization of Real-Time PCR Data in Calliptamus italicus (Orthoptera: Acrididae) Under Different Temperature Conditions.Journal of insect science (Online) · 2019Article
- Identification and validation of potential reference gene for effective dsRNA knockdown analysis in Chilo partellus.Scientific reports · 2019Article
- Evaluation and Validation of Reference Genes for Quantitative Real-Time PCR in Helopeltis theivora Waterhouse (Hemiptera: Miridae).Scientific reports · 2019Article
- Evaluation of reference genes for real-time quantitative PCR analysis in southern corn rootworm, Diabrotica undecimpunctata howardi (Barber).Scientific reports · 2019Article
- Reference gene selection for quantitative gene expression analysis in black soldier fly (Hermetia illucens).PloS one · 2019Article
- Selection of reference genes for expression analysis of plant-derived microRNAs in Plutella xylostella using qRT-PCR and ddPCR.PloS one · 2019Article
- Selection and validation of reference genes desirable for gene expression analysis by qRT-PCR in MeJA-treated ginseng hairy roots.PloS one · 2019Article
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Abstract
The Bemisia tabaci Mediterranean (MED) cryptic species has been rapidly invading to most parts of the world owing to its strong ecological adaptability, which is considered as a model insect for stress tolerance studies under rapidly changing environments. Selection of a suitable reference gene for quantitative stress-responsive gene expression analysis based on qRT-PCR is critical for elaborating the molecular mechanisms of thermotolerance. To obtain accurate and reliable normalization data in MED, eight candidate reference genes (β-act, GAPDH, β-tub, EF1-α, GST, 18S, RPL13A and α-tub) were examined under various thermal stresses for varied time periods by using geNorm, NormFinder and BestKeeper algorithms, respectively. Our results revealed that β-tub and EF1-α were the best reference genes across all sample sets. On the other hand, 18S and GADPH showed the least stability for all the samples studied. β-act was proved to be highly stable only in case of short-term thermal stresses. To our knowledge this was the first comprehensive report on validation of reference genes under varying temperature stresses in MED. The study could expedite particular discovery of thermotolerance genes in MED. Further, the present results can form the basis of further research on suitable reference genes in this invasive insect and will facilitate transcript profiling in other invasive insects.
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