Evidence map›Paper›PMID 28322577›Full record

ArticleEpigenomics2017

BET proteins are a key component of immunoglobulin gene expression.

Jung Min Shim, Jin S Lee, Kirsty E Russell, Coen H Wiegman, Peter J Barnes, David Fear, Ian M Adcock, Andrew L Durham

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Epigenomics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.6field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 11 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. Review
  5. [Mechanism of action of BET bromodomain inhibitor JQ1 in treating airway remodeling in asthmatic mice].Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics · 2017
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Jung Min ShimAirways Disease Section, National Heart & Lung Institute, Imperial College London, London, SW7 2AZ, UK.
Jin S LeeAirways Disease Section, National Heart & Lung Institute, Imperial College London, London, SW7 2AZ, UK.
Kirsty E RussellAirways Disease Section, National Heart & Lung Institute, Imperial College London, London, SW7 2AZ, UK.
Coen H WiegmanAirways Disease Section, National Heart & Lung Institute, Imperial College London, London, SW7 2AZ, UK.
Peter J BarnesAirways Disease Section, National Heart & Lung Institute, Imperial College London, London, SW7 2AZ, UK.
David FearMRC & Asthma UK Centre in Allergic Mechanisms of Asthma, Department of Respiratory Medicine & Allergy, King's College London, London, WC2R 2LS, UK.
Ian M AdcockAirways Disease Section, National Heart & Lung Institute, Imperial College London, London, SW7 2AZ, UK.
Andrew L DurhamAirways Disease Section, National Heart & Lung Institute, Imperial College London, London, SW7 2AZ, UK.
Imperial College London · GBKing's College London · GB

Funding

Medical Research Council G0801266Medical Research Council G1000758
6 · The paper itself

Abstract

aimBET proteins have been shown to regulate gene expression including inflammatory genes.

methodsIn order to investigate the role of the BET proteins in immunoglobulin production we treated the human B-cell line CLNH11.4 and primary human B cells and ozone-exposed mice with BET inhibitors (JQ1 or IBET151).

resultsBoth proliferation and IgG production were reduced by JQ1 in a concentration-dependent manner. JQ1 significantly reduced immunoglobulin gene transcription. In vivo treatment of ozone-exposed mice with the BET inhibitor IBET151 similarly inhibited ozone-induced immunoglobulin production. JQ1 did not reduce the protein levels of Brd4 or Oct2 per se but reduced the ability of Brd4 and Oct2 to co-immunoprecipitate and of Oct2 to bind to immunoglobulin gene promoters.

conclusionOur results indicate that BET proteins including Brd4 play a crucial role regulation B-cell-specific gene expression and immunoglobulin production.

Indexed as

AnimalsAzepinesB-LymphocytesBromodomain Containing ProteinsCell Cycle ProteinsCell LineCell ProliferationDose-Response Relationship, DrugEpigenesis, GeneticGene Expression RegulationHeterocyclic Compounds, 4 or More RingsHumansImmunoglobulin GMiceNuclear ProteinsOrganic Cation Transporter 2AzepinesBRD4 protein, humanBromodomain Containing ProteinsCell Cycle ProteinsGSK1210151AHeterocyclic Compounds, 4 or More RingsImmunoglobulin G(+)-JQ1 compoundNuclear ProteinsOrganic Cation Transporter 2Organic Cation Transport ProteinsSLC22A2 protein, humanTranscription FactorsTriazolesB cellsBrd4bromo and extraterminal domainsepigenetic gene regulationhistone modificationsimmunoglobulinJQ1Oct2

Identifiers

PMID28322577
PMCPMC5476213
OpenAlexW2599783854

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.