ArticleScientific reports2017
Imaging tissue-mimic with light sheet microscopy: A comparative guideline.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Integration of multi-modal monitoring for dynamic control of large-scale 3D tissue bioreactors.Scientific reports · 2026Article
- A versatile and open source design for one and two photon light sheet microscopy.Scientific reports · 2025Article
- Article
- Successful 3D imaging of cleared biological samples with light sheet fluorescence microscopy.The Journal of cell biology · 2023Review
- HIV-1 transmission: modelling and direct visualization in the third dimension.Microscopy (Oxford, England) · 2023Review
- Single-Cell and Spatial Analysis of Emergent Organoid Platforms.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Article
- Multiple asters organize the yolk microtubule network during dclk2-GFP zebrafish epiboly.Scientific reports · 2022Article
- Current and future applications of induced pluripotent stem cell-based models to study pathological proteins in neurodegenerative disorders.Molecular psychiatry · 2021Review
- Head-to-Head Comparison of the Penetration Efficiency of Lipid-Based Nanoparticles into Tumor Spheroids.ACS omega · 2020Article
- Article
- Ultra-thin fluorocarbon foils optimise multiscale imaging of three-dimensional native and optically cleared specimens.Scientific reports · 2019Article
- Highly Sensitive Shack-Hartmann Wavefront Sensor: Application to Non-Transparent Tissue Mimic Imaging with Adaptive Light-Sheet Fluorescence Microscopy.Methods and protocols · 2019Article
- Enhanced Light Sheet Elastic Scattering Microscopy by Using a Supercontinuum Laser.Methods and protocols · 2019Article
- 3D Cell-Based Assays for Drug Screens: Challenges in Imaging, Image Analysis, and High-Content Analysis.SLAS discovery : advancing life sciences R & D · 2019Article
- Fast 3-D Imaging of Brain Organoids With a New Single-Objective Planar-Illumination Two-Photon Microscope.Frontiers in neuroanatomy · 2019Article
- Cancellation of Bessel beam side lobes for high-contrast light sheet microscopy.Scientific reports · 2018Article
- Improved contrast in inverted selective plane illumination microscopy of thick tissues using confocal detection and structured illumination.Biomedical optics express · 2017Article
Corrections and comments
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tissue mimics (TMs) on the scale of several hundred microns provide a beneficial cell culture configuration for in vitro engineered tissue and are currently under the spotlight in tissue engineering and regenerative medicine. Due to the cell density and size, TMs are fairly inaccessible to optical observation and imaging within these samples remains challenging. Light Sheet Fluorescence Microscopy (LSFM)- an emerging and attractive technique for 3D optical sectioning of large samples- appears to be a particularly well-suited approach to deal with them. In this work, we compared the effectiveness of different light sheet illumination modalities reported in the literature to improve resolution and/or light exposure for complex 3D samples. In order to provide an acute and fair comparative assessment, we also developed a systematic, computerized benchmarking method. The outcomes of our experiment provide meaningful information for valid comparisons and arises the main differences between the modalities when imaging different types of TMs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.