ArticleCytotechnology2017
Colony, hanging drop, and methylcellulose three dimensional hypoxic growth optimization of renal cell carcinoma cell lines.
Article in Cytotechnology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Optimization of 3D Spheroid Models of Ovarian, Breast, and Gastric Cancer for Comparative Screening of Drug Candidates.ACS omega · 2026Article
- Using ovine eyes as a readily available source for the isolation and 2D or 3D cultivation of uveal melanocytes.PloS one · 2026Article
- Three-Dimensional Tumor Spheroids of Pancreatic and Colorectal Cancer Cell Lines for High-Throughput Screening of Drug Candidates.ACS omega · 2025Article
- Fabrication of a Three-Dimensional Spheroid Culture System for Oral Squamous Cell Carcinomas Using a Microfabricated Device.Cancers · 2023Article
- Combinatorial drug screening on 3D Ewing sarcoma spheroids using droplet-based microfluidics.iScience · 2023Article
- Looking into the Eyes-In Vitro Models for Ocular Research.International journal of molecular sciences · 2022Review
- PFKFB3 regulates cancer stemness through the hippo pathway in small cell lung carcinoma.Oncogene · 2022Article
- Spheroid culture models adequately imitate distinctive features of the renal cancer or melanoma microenvironment.In vitro cellular & developmental biology. Animal · 2022Article
- Article
- A Single-Cell Culture System for Dissecting Microenvironmental Signaling in Development and Disease of Cartilage Tissue.Frontiers in cell and developmental biology · 2021Article
- Role of cellulose family in fibril organization of collagen for forming 3D cancer spheroids:BioImpacts : BI · 2021Article
- Maintenance of viability and proliferation of 3T3 cell aggregates incorporating fibroin microspheres into cultures.Cytotechnology · 2020Article
- A comparative study of the capacity of mesenchymal stromal cell lines to form spheroids.PloS one · 2020Article
- Expression and epigenetic regulatory mechanism of BNIP3 in clear cell renal cell carcinoma.International journal of oncology · 2019Article
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Authors and funding
6 authors.
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Abstract
Renal cell carcinoma (RCC) is the most lethal of the common urologic malignancies, comprising 3% of all human neoplasms, and the incidence of kidney cancer is rising annually. We need new approaches to target tumor cells that are resistant to current therapies and that give rise to recurrence and treatment failure. In this study, we focused on low oxygen tension and three-dimensional (3D) cell culture incorporation to develop a new RCC growth model. We used the hanging drop and colony formation methods, which are common in 3D culture, as well as a unique methylcellulose (MC) method. For the experiments, we used human primary RCC cell lines, metastatic RCC cell lines, human kidney cancer stem cells, and human healthy epithelial cells. In the hanging drop assay, we verified the potential of various cell lines to create solid aggregates in hypoxic and normoxic conditions. With the semi-soft agar method, we also determined the ability of various cell lines to create colonies under different oxygen conditions. Different cell behavior observed in the MC method versus the hanging drop and colony formation assays suggests that these three assays may be useful to test various cell properties. However, MC seems to be a particularly valuable alternative for 3D cell culture, as its higher efficiency of aggregate formation and serum independency are of interest in different areas of cancer biology.
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