Evidence map›Paper›PMID 28320593›Full record

ArticleDNA repair2017

Spore photoproduct within DNA is a surprisingly poor substrate for its designated repair enzyme-The spore photoproduct lyase.

Linlin Yang, Yajun Jian, Peter Setlow, Lei Li

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in DNA repair, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.6field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 8 citations in OpenAlex.

  1. Decoupling Activity and Specificity in Coronazymes.Small (Weinheim an der Bergstrasse, Germany) · 2025
    Article
  2. Article
  3. Application of a Krypton-Chlorine Excilamp To ControlApplied and environmental microbiology · 2020
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Linlin YangDepartment of Chemistry and Chemical Biology, Indiana University-Purdue University Indianapolis (IUPUI), 402 North Blackford Street, Indianapolis, IN 46202, United States.
Yajun JianDepartment of Chemistry and Chemical Biology, Indiana University-Purdue University Indianapolis (IUPUI), 402 North Blackford Street, Indianapolis, IN 46202, United States.
Peter SetlowDepartment of Molecular Biology and Biophysics, UConn Health, Farmington, CT 06030, United States.
Lei LiDepartment of Chemistry and Chemical Biology, Indiana University-Purdue University Indianapolis (IUPUI), 402 North Blackford Street, Indianapolis, IN 46202, United States; Department of Dermatology, Indiana University School of Medicine, Indianapolis, IN 46202, United States. Electronic address: lilei@iupui.edu.
Indiana University – Purdue University Indianapolis · USUConn Health · US

Funding

Enzyme Catalysis of Toluene Degradation and Unusual DNA Photoproduct RepairR00ES017177 · NIEHS · INDIANA UNIVERSITY INDIANAPOLIS · PI LI, LEI · 2009 to 2011
$747k
Enzyme Catalysis of Toluene Degradation and Unusual DNA Photoproduct RepairK99ES017177 · NIEHS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI LI, LEI · 2008 to 2009
$167k
NIEHS NIH HHS K99 ES017177NIEHS NIH HHS R00 ES017177
6 · The paper itself

Abstract

DNA repair enzymes typically recognize their substrate lesions with high affinity to ensure efficient lesion repair. In UV irradiated endospores, a special thymine dimer, 5-thyminyl-5,6-dihydrothymine, termed the spore photoproduct (SP), is the dominant DNA photolesion, which is rapidly repaired during spore outgrowth mainly by spore photoproduct lyase (SPL) using an unprecedented protein-harbored radical transfer process. Surprisingly, our in vitro studies using SP-containing short oligonucleotides, pUC 18 plasmid DNA, and E. coli genomic DNA found that they are all poor substrates for SPL in general, exhibiting turnover numbers of 0.01-0.2min

Indexed as

DNA RepairBacillus subtilisBacterial ProteinsDeoxyribodipyrimidine Photo-LyaseDNA, BacterialDNA DamageEscherichia coliKineticsPyrimidine DimersThymineUltraviolet Rays5-thyminyl-5,6-dihydrothymineBacterial ProteinsDeoxyribodipyrimidine Photo-LyaseDNA, BacterialPyrimidine DimersThymineDirect reversalDNA photolesionsDNA repairEndosporesRadical SAM enzymesRadical transferSpore photoproductThymine dimer

Identifiers

PMID28320593
PMCPMC5654379
OpenAlexW2592074016

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.