Evidence map›Paper›PMID 28320483›Full record

ArticleStem cell research & therapy2017

Human and feline adipose-derived mesenchymal stem cells have comparable phenotype, immunomodulatory functions, and transcriptome.

Kaitlin C Clark, Fernando A Fierro, Emily Mills Ko, Naomi J Walker, Boaz Arzi, Clifford G Tepper, Heather Dahlenburg, Andrew Cicchetto, Amir Kol, Lyndsey Marsh and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell research & therapy, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed
4.3field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed, 52 citations in OpenAlex.

  1. Trial
  2. The Biodistribution ofVeterinary sciences · 2026
    Article
  3. Review
  4. Article
  5. Article
  6. Review
  7. Review
  8. Review
  9. Isolation and characterization of feline dental pulp stem cells.Journal of feline medicine and surgery · 2023
    Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Review
  15. An Update on Feline Chronic Gingivostomatitis.The Veterinary clinics of North America. Small animal practice · 2020
    Review
  16. Article
  17. Article
  18. Adipose-Derived Stromal Cells Seeded on IntegraBioengineering (Basel, Switzerland) · 2020
    Article
  19. Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 3 institutions in 1 country.

Kaitlin C ClarkVeterinary Institute for Regenerative Cures and Department of Pathology, Microbiology and Immunology, University of California, Davis, CA, 95816, USA.
Fernando A FierroInstitute for Regenerative Cures and Department of Cell Biology and Human Anatomy, University of California, Davis, CA, 95816, USA.
Emily Mills KoVeterinary Institute for Regenerative Cures and Department of Pathology, Microbiology and Immunology, University of California, Davis, CA, 95816, USA.
Naomi J WalkerVeterinary Institute for Regenerative Cures and Department of Pathology, Microbiology and Immunology, University of California, Davis, CA, 95816, USA.
Boaz ArziDepartment of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Davis, CA, 95816, USA.
Clifford G TepperDepartment of Biochemistry and Molecular Medicine, University of California, Davis, CA, 95816, USA.
Heather DahlenburgInstitute for Regenerative Cures and Department of Cell Biology and Human Anatomy, University of California, Davis, CA, 95816, USA.
Andrew CicchettoInstitute for Regenerative Cures and Department of Cell Biology and Human Anatomy, University of California, Davis, CA, 95816, USA.
Amir KolVeterinary Institute for Regenerative Cures and Department of Pathology, Microbiology and Immunology, University of California, Davis, CA, 95816, USA.
Lyndsey MarshVeterinary Institute for Regenerative Cures and Department of Pathology, Microbiology and Immunology, University of California, Davis, CA, 95816, USA.
William J MurphyDepartment of Dermatology, School of Medicine, University of California, Davis, CA, 95816, USA.
Nasim FazelDepartment of Dermatology, School of Medicine, University of California, Davis, CA, 95816, USA.
Dori L BorjessonVeterinary Institute for Regenerative Cures and Department of Pathology, Microbiology and Immunology, University of California, Davis, CA, 95816, USA. dlborjesson@ucdavis.edu.
University of California, Davis · USCalifornia Institute for Regenerative Medicine · USVeterinary Medical Teaching Hospital · US

Funding

Staff InvestigatorsP30CA093373 · NCI · UNIVERSITY OF CALIFORNIA DAVIS · PI KC KENT LLOYD · 2002 to 2026
$84.9M
An Animal Model of Chronic Oral Inflammation for Stem Cell-Based TherapyR21DE024711 · NIDCR · UNIVERSITY OF CALIFORNIA AT DAVIS · PI BORJESSON, DORI L. · 2014 to 2015
$312k
NCI NIH HHS P30 CA093373NIDCR NIH HHS R21 DE024711
6 · The paper itself

Abstract

backgroundAdipose-derived mesenchymal stem cells (ASCs) are a promising cell therapy to treat inflammatory and immune-mediated diseases. Development of appropriate pre-clinical animal models is critical to determine safety and attain early efficacy data for the most promising therapeutic candidates. Naturally occurring diseases in cats already serve as valuable models to inform human clinical trials in oncologic, cardiovascular, and genetic diseases. The objective of this study was to complete a comprehensive side-by-side comparison of human and feline ASCs, with an emphasis on their immunomodulatory capacity and transcriptome.

methodsHuman and feline ASCs were evaluated for phenotype, immunomodulatory profile, and transcriptome. Additionally, transwells were used to determine the role of cell-cell contact in ASC-mediated inhibition of lymphocyte proliferation in both humans and cats.

resultsSimilar to human ASCs, feline ASCs were highly proliferative at low passages and fit the minimal criteria of multipotent stem cells including a compatible surface protein phenotype, osteogenic capacity, and normal karyotype. Like ASCs from all species, feline ASCs inhibited mitogen-activated lymphocyte proliferation in vitro, with or without direct ASC-lymphocyte contact. Feline ASCs mimic human ASCs in their mediator secretion pattern, including prostaglandin E2, indoleamine 2,3 dioxygenase, transforming growth factor beta, and interleukin-6, all augmented by interferon gamma secretion by lymphocytes. The transcriptome of three unactivated feline ASC lines were highly similar. Functional analysis of the most highly expressed genes highlighted processes including: 1) the regulation of apoptosis; 2) cell adhesion; 3) response to oxidative stress; and 4) regulation of cell differentiation. Finally, feline ASCs had a similar gene expression profile to noninduced human ASCs.

conclusionsFindings suggest that feline ASCs modulate lymphocyte proliferation using soluble mediators that mirror the human ASC secretion pattern. Uninduced feline ASCs have similar gene expression profiles to uninduced human ASCs, as revealed by transcriptome analysis. These data will help inform clinical trials using cats with naturally occurring diseases as surrogate models for human clinical trials in the regenerative medicine arena.

Indexed as

Adipose TissueAnimalsCatsCell ProliferationCell ShapeFemaleGene Expression ProfilingHumansImmunologic FactorsImmunomodulationInflammation MediatorsMesenchymal Stem CellsMitogensPhenotypeT-LymphocytesTranscriptomeImmunologic FactorsInflammation MediatorsMitogensAdipose tissueAnimal modelFelineHumanImmunomodulationMesenchymal stem cellMultipotent adult progenitor cell

Identifiers

PMID28320483
PMCPMC5360077
OpenAlexW2602258022

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.