Evidence map›Paper›PMID 28319648›Full record

ArticleProteomics2017

New targeted approaches for the quantification of data-independent acquisition mass spectrometry.

Roland Bruderer, Julia Sondermann, Chih-Chiang Tsou, Alonso Barrantes-Freer, Christine Stadelmann, Alexey I Nesvizhskii, Manuela Schmidt, Lukas Reiter, David Gomez-Varela

Abstract read
In one paragraph

Article in Proteomics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. diaTracer enables spectrum-centric analysis of diaPASEF proteomics data.bioRxiv : the preprint server for biology · 2024
    Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Transcriptomic and proteomic profiling of NaFrontiers in molecular neuroscience · 2022
    Article
  13. Article
  14. Review
  15. Review
  16. Article
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Roland BrudererBiognosys AG, Schlieren, Switzerland.
Julia SondermannSomatosensory Signaling and Systems Biology Research Group, Max Planck Institute of Experimental Medicine, Goettingen, Germany.
Chih-Chiang TsouDepartment of Computational Medicine and Bioinformatics, University of Michigan, Ann Arbor, MI, USA.
Alonso Barrantes-FreerInstitute of Neuropathology, University Medical Center, Goettingen, Germany.
Christine StadelmannInstitute of Neuropathology, University Medical Center, Goettingen, Germany.
Alexey I NesvizhskiiDepartment of Computational Medicine and Bioinformatics, University of Michigan, Ann Arbor, MI, USA.
Manuela SchmidtSomatosensory Signaling and Systems Biology Research Group, Max Planck Institute of Experimental Medicine, Goettingen, Germany.
Lukas ReiterBiognosys AG, Schlieren, Switzerland.
David Gomez-VarelaSomatosensory Signaling and Systems Biology Research Group, Max Planck Institute of Experimental Medicine, Goettingen, Germany.

Funding

COMPUTATIONAL TOOLS FOR MASS SPECTROMETRY-BASED INTERACTOME DATAR01GM094231 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Alexey I Nesvizhskii · 2010 to 2026
$5.4M
NIGMS NIH HHS R01 GM094231
6 · The paper itself

Abstract

The use of data-independent acquisition (DIA) approaches for the reproducible and precise quantification of complex protein samples has increased in the last years. The protein information arising from DIA analysis is stored in digital protein maps (DIA maps) that can be interrogated in a targeted way by using ad hoc or publically available peptide spectral libraries generated on the same sample species as for the generation of the DIA maps. The restricted availability of certain difficult-to-obtain human tissues (i.e., brain) together with the caveats of using spectral libraries generated under variable experimental conditions limits the potential of DIA. Therefore, DIA workflows would benefit from high-quality and extended spectral libraries that could be generated without the need of using valuable samples for library production. We describe here two new targeted approaches, using either classical data-dependent acquisition repositories (not specifically built for DIA) or ad hoc mouse spectral libraries, which enable the profiling of human brain DIA data set. The comparison of our results to both the most extended publically available human spectral library and to a state-of-the-art untargeted method supports the use of these new strategies to improve future DIA profiling efforts.

Indexed as

SoftwareAnimalsComputational BiologyHumansMass SpectrometryMicePeptide LibraryPrefrontal CortexProteomeProteomicsSpinal CordPeptide LibraryProteomeData-independent acquisitionSpectral libraries

Identifiers

PMID28319648
PMCPMC5870755

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.