ArticlePloS one2017
A digital PCR method for identifying and quantifying adulteration of meat species in raw and processed food.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 93 citations in OpenAlex.
- Meta-analysis of variable-temperature PCR technique performance for diagnosising Schistosoma japonicum infections in humans in endemic areas.PLoS neglected tropical diseases · 2022Pooled it
- Detection and Quantification of House Crickets (Insects · 2025Article
- Droplet Digital PCR (ddPCR) for Halal Detection of Commercial Gelatin Products.International journal of food science · 2025Article
- Analysis of species adulteration in beef sausage using real-time polymerase chain reaction in Makassar, Indonesia.Veterinary world · 2024Article
- DNA Metabarcoding Approach as a Potential Tool for Supporting Official Food Control Programs: A Case Study.Foods (Basel, Switzerland) · 2024Article
- A multiplex DNA probe-based method for simultaneous identification of adulteration in meat samples.Food chemistry. Molecular sciences · 2024Article
- Article
- A Droplet Digital PCR-Based Approach for Quantitative Analysis of the Adulteration of Atlantic Salmon with Rainbow Trout.Foods (Basel, Switzerland) · 2023Article
- Development of Seven New dPCR Animal Species Assays and a Reference Material to Support Quantitative Ratio Measurements of Food and Feed Products.Foods (Basel, Switzerland) · 2023Article
- Article
- A Droplet Digital PCR Based Approach for Identification and Quantification of Porcine and Chicken Derivatives in Beef.Foods (Basel, Switzerland) · 2022Article
- A dPCR Method for Quantitative Authentication of Wild Lingonberry (Foods (Basel, Switzerland) · 2022Article
- A quantitative detection of mung bean in chestnut paste using duplex digital PCR.Current research in food science · 2022Article
- DNA Accounting: Tallying Genomes to Detect Adulterated Saffron.Foods (Basel, Switzerland) · 2021Article
- Omics-Based Analytical Approaches for Assessing Chicken Species and Breeds in Food Authentication.Molecules (Basel, Switzerland) · 2021Review
- Quantification of Actaea racemosa L. (black cohosh) from some of its potential adulterants using qPCR and dPCR methods.Scientific reports · 2021Article
- Current Trends of Food Analysis, Safety, and Packaging.International journal of food science · 2021Review
- Precise Populations' Description in Dairy Ecosystems Using Digital Droplet PCR: The Case ofFrontiers in microbiology · 2020Article
- Detection of meat from horse, donkey and their hybrids (mule/hinny) by duplex real-time fluorescent PCR.PloS one · 2020Article
- Detection of fish allergen by droplet digital PCR.Italian journal of food safety · 2018Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Meat adulteration is a worldwide concern. In this paper, a new droplet digital PCR (ddPCR) method was developed for the quantitative determination of the presence of chicken in sheep and goat meat products. Meanwhile, a constant (multiplication factor) was introduced to transform the ratio of copy numbers to the proportion of meats. The presented ddPCR method was also proved to be more accurate (showing bias of less than 9% in the range from 5% to 80%) than real-time PCR, which has been widely used in this determination. The method exhibited good repeatability and stability in different thermal treatments and at ultra-high pressure. The relative standard deviation (RSD) values of 5% chicken content was less than 5.4% for ultra-high pressure or heat treatment. Moreover, we confirmed that different parts of meat had no effect on quantification accuracy of the ddPCR method. In contrast to real-time PCR, we examined the performance of ddPCR as a more precise, sensitive and stable analytical strategy to overcome potential problems of discrepancies in amplification efficiency discrepancy and to obtain the copy numbers directly without standard curves. The method and strategy developed in this study can be applied to quantify the presence and to confirm the absence of adulterants not only to sheep but also to other kinds of meat and meat products.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.