ArticlePloS one2017
Structural insights into the regulation of Bacillus subtilis SigW activity by anti-sigma RsiW.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Discovery of Novel c-di-GMP-Related Genes inPathogens (Basel, Switzerland) · 2026Article
- Article
- Structural basis for regulation of a CBASS-CRISPR-Cas defense island by a transmembrane anti-σ factor and its ECF σ partner.Science advances · 2024Article
- In Silico Safety Assessment ofInternational journal of molecular sciences · 2024Article
- Structural Analysis ofMicroorganisms · 2023Article
- The Burkholderia cenocepacia iron starvation σ factor, OrbS, possesses an on-board iron sensor.Nucleic acids research · 2022Article
- Cell Envelope Stress Response in Pseudomonas aeruginosa.Advances in experimental medicine and biology · 2022Article
- Extracytoplasmic Function σ Factors as Tools for Coordinating Stress Responses.International journal of molecular sciences · 2021Review
- Diverse and unified mechanisms of transcription initiation in bacteria.Nature reviews. Microbiology · 2021Review
- Mini Review: Bacterial Membrane Composition and Its Modulation in Response to Stress.Frontiers in molecular biosciences · 2021Review
- Autonomous and Assisted Control for Synthetic Microbiology.International journal of molecular sciences · 2020Review
- Article
- Metal-responsive RNA polymerase extracytoplasmic function (ECF) sigma factors.Molecular microbiology · 2019Review
- The pneumococcal σThe Journal of biological chemistry · 2019Article
- Structural analysis of the recognition of the -35 promoter element by SigW from Bacillus subtilis.PloS one · 2019Article
- The crystal structure of the RsbN-σBldN complex from Streptomyces venezuelae defines a new structural class of anti-σ factor.Nucleic acids research · 2018Article
- Themes and variations in gene regulation by extracytoplasmic function (ECF) sigma factors.Current opinion in microbiology · 2017Review
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacillus subtilis SigW is localized to the cell membrane and is inactivated by the tight interaction with anti-sigma RsiW under normal growth conditions. Whereas SigW is discharged from RsiW binding and thus initiates the transcription of its regulon under diverse stress conditions such as antibiotics and alkaline shock. The release and activation of SigW in response to extracytoplasmic signals is induced by the regulated intramembrane proteolysis of RsiW. As a ZAS (Zinc-containing anti-sigma) family protein, RsiW has a CHCC zinc binding motif, which implies that its anti-sigma activity may be regulated by the state of zinc coordination in addition to the proteolytic cleavage of RsiW. To understand the regulation mode of SigW activity by RsiW, we determined the crystal structures of SigW in complex with the cytoplasmic domain of RsiW, and compared the conformation of the CHCC motif in the reduced/zinc binding and the oxidized states. The structures revealed that RsiW inhibits the promoter binding of SigW by interacting with the surface groove of SigW. The interaction between SigW and RsiW is not disrupted by the oxidation of the CHCC motif in RsiW, suggesting that SigW activity might not be regulated by the zinc coordination states of the CHCC motif.
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