ArticlePharmaceutical biology2017
Bioactivity guided isolation of cytotoxic terpenoids and steroids from Premna serratifolia.
Article in Pharmaceutical biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 16 citations in OpenAlex.
- Integrated Phytochemical and Pharmacological Insights intoPlants (Basel, Switzerland) · 2026Review
- Isolation of Natural Products Using Preparative TLC.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Discovery and Biosynthesis of a Structurally Dynamic Antibacterial Diterpenoid.Angewandte Chemie (International ed. in English) · 2021Article
- Cytotoxic and DNA-Damaging Effects ofAntioxidants (Basel, Switzerland) · 2020Article
- Silver Nanoparticle Production byBioinorganic chemistry and applications · 2020Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
contextDespite several phytochemical studies of Premna serratifolia Linn. (Verbenaceae), the isolation of active constituents of this plant remains to be explored.
objectiveThe study isolates cytotoxic terpenoids and steroids from the leaves of Premna serratifolia. MATERIALS AND
methodsUnsaponifiable matter of hexane soluble fraction obtained from methanol extract was subjected to isolation by column chromatography and preparative TLC. Three compounds PS-01 A, PS-01B and PS-02 A were isolated. PS-01 A and PS-01B were identified by comparative TLC with authentic marker compounds followed by NMR analysis. Further PS-01B was analyzed by HR-GCMS. PS-02 A was subjected to HR-LCMS. All isolated compounds/fractions were evaluated for cytotoxic activity by BSL bioassay and using cell lines A549, HepG2 and L6.
resultsThree compounds were isolated from the leaf extract by bioactivity-guided fractionation. Two of which, namely, PS-01 A (oleanolic acid) and PS-02 A (unknown) were found to be terpenoids and PS-01B was identified as steroid (stigmasterol). PS-02 A compound is to be purified and characterized further. All three compounds PS-01 A, PS-01B, PS-02 A showed cytotoxicity by BSL bioassay (LC
conclusionBioactivity guided fractionation of Premna serratifolia leaves succeeded into isolation of two terpenoids and one steroid compound with significant cytotoxic activity. Here we report the isolation of these cytotoxic terpenoids/steroids from this plant for the first time which could be developed as anticancer agents.
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