ArticleThe Journal of biological chemistry2017
Spatial organization of DNA sequences directs the assembly of bacterial chromatin by a nucleoid-associated protein.
Article in The Journal of biological chemistry, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Deciphering Sequence-Specific DNA Binding by H-NS Using Molecular Simulation.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Decoding dissociation of sequence-specific protein-DNA complexes with non-equilibrium simulations.Nucleic acids research · 2023Article
- Facilitated dissociation of nucleoid-associated proteins from DNA in the bacterial confinement.Biophysical journal · 2022Article
- Molecular Basis for Environment Sensing by a Nucleoid-Structuring Bacterial Protein Filament.The journal of physical chemistry letters · 2021Article
- Nucleoid-associated proteins shape chromatin structure and transcriptional regulation across the bacterial kingdom.Transcription · 2021Review
- Article
- Hypothesis: nucleoid-associated proteins segregate with a parental DNA strand to generate coherent phenotypic diversity.Theory in biosciences = Theorie in den Biowissenschaften · 2021Article
- Genome-in-a-Box: Building a Chromosome from the Bottom Up.ACS nano · 2021Review
- Redefining the H-NS protein family: a diversity of specialized core and accessory forms exhibit hierarchical transcriptional network integration.Nucleic acids research · 2020Article
- Chromosomal Organization and Regulation of Genetic Function inEcoSal Plus · 2020Article
- Different Proteins Mediate Step-Wise Chromosome Architectures inFrontiers in microbiology · 2020Article
- Coherent Domains of Transcription Coordinate Gene Expression During Bacterial Growth and Adaptation.Microorganisms · 2019Review
- The extruded non-template strand determines the architecture of R-loops.Nucleic acids research · 2019Article
- Direct imaging of the circular chromosome in a live bacterium.Nature communications · 2019Article
- Predicting the mechanism and rate of H-NS binding to AT-rich DNA.PLoS computational biology · 2019Article
- Article
- Revised role for Hfq bacterial regulator on DNA topology.Scientific reports · 2018Article
- Chromosomal organization of transcription: in a nutshell.Current genetics · 2018Review
- Impact of Chromosomal Architecture on the Function and Evolution of Bacterial Genomes.Frontiers in microbiology · 2018Review
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7 authors.
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Abstract
Structural differentiation of bacterial chromatin depends on cooperative binding of abundant nucleoid-associated proteins at numerous genomic DNA sites and stabilization of distinct long-range nucleoprotein structures. Histone-like nucleoid-structuring protein (H-NS) is an abundant DNA-bridging, nucleoid-associated protein that binds to an AT-rich conserved DNA sequence motif and regulates both the shape and the genetic expression of the bacterial chromosome. Although there is ample evidence that the mode of H-NS binding depends on environmental conditions, the role of the spatial organization of H-NS-binding sequences in the assembly of long-range nucleoprotein structures remains unknown. In this study, by using high-resolution atomic force microscopy combined with biochemical assays, we explored the formation of H-NS nucleoprotein complexes on circular DNA molecules having different arrangements of identical sequences containing high-affinity H-NS-binding sites. We provide the first experimental evidence that variable sequence arrangements result in various three-dimensional nucleoprotein structures that differ in their shape and the capacity to constrain supercoils and compact the DNA. We believe that the DNA sequence-directed versatile assembly of periodic higher-order structures reveals a general organizational principle that can be exploited for knowledge-based design of long-range nucleoprotein complexes and purposeful manipulation of the bacterial chromatin architecture.
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