Evidence map›Paper›PMID 28315936›Full record

ArticleImmunogenetics2017

Peptide-binding motifs of two common equine class I MHC molecules in Thoroughbred horses.

Tobias Bergmann, Mikaela Lindvall, Erin Moore, Eugene Moore, John Sidney, Donald Miller, Rebecca L Tallmadge, Paisley T Myers, Stacy A Malaker, Jeffrey Shabanowitz and 5 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Immunogenetics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact, top 98% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 5 institutions in 2 countries.

Tobias BergmannInstitut für Virologie, Freie Universität Berlin, 14163, Berlin, Germany.
Mikaela LindvallDepartment of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, 9420 Athena Circle, La Jolla, CA, 92037, USA.
Erin MooreDepartment of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, 9420 Athena Circle, La Jolla, CA, 92037, USA.
Eugene MooreDepartment of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, 9420 Athena Circle, La Jolla, CA, 92037, USA.
John SidneyDepartment of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, 9420 Athena Circle, La Jolla, CA, 92037, USA.
Donald MillerBaker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, NY, 14853, USA.
Rebecca L TallmadgeDepartment of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, 14853, USA.
Paisley T MyersDepartment of Chemistry, University of Virginia, Charlottesville, VA, 22904, USA.
Stacy A MalakerDepartment of Chemistry, University of Virginia, Charlottesville, VA, 22904, USA.
Jeffrey ShabanowitzDepartment of Chemistry, University of Virginia, Charlottesville, VA, 22904, USA.
Nikolaus OsterriederInstitut für Virologie, Freie Universität Berlin, 14163, Berlin, Germany.
Bjoern PetersDepartment of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, 9420 Athena Circle, La Jolla, CA, 92037, USA.
Donald F HuntDepartment of Chemistry, University of Virginia, Charlottesville, VA, 22904, USA.
Douglas F AntczakBaker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, NY, 14853, USA.
Alessandro SetteDepartment of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, 9420 Athena Circle, La Jolla, CA, 92037, USA. alex@lji.org.
La Jolla Institute for Immunology · USUniversity of Virginia · USCornell University · USFreie Universität Berlin · DENew York State College of Veterinary Medicine · US

Funding

Systems Biological Analysis of Innate and Adaptive Responses to VaccinationU19AI090023 · NIAID · EMORY UNIVERSITY · PI AHMED, RAFI, LI, SHUZHAO · 2010 to 2021
$45.0M
PROCESSED ANTIGEN CHARACTERIZATION BY MASS SPECTROMETRYR37AI033993 · NIAID · UNIVERSITY OF VIRGINIA CHARLOTTESVILLE · PI HUNT, DONALD F · 1999 to 2008
$5.6M
PROCESSED ANTIGEN CHARACTERIZATION BY MASS SPECTROMETRYR01AI033993 · NIAID · UNIVERSITY OF VIRGINIA CHARLOTTESVILLE · PI HUNT, DONALD F · 1994 to 2017
$4.9M
A Rational Approach for HIV Vaccine T Cell Epitope SelectionR01AI112566 · NIAID · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI GOEPFERT, PAUL A. · 2014 to 2017
$1.9M
Emory R38 Research Training ProgramR38AI140299 · NIAID · EMORY UNIVERSITY · PI OFOTOKUN, IGHOVWERHA, ROUPHAEL, NADINE GEORGES · 2018 to 2021
$1.4M
NIAID NIH HHS HHSN272200900042CNIAID NIH HHS HHSN272201400045CNIAID NIH HHS R01 AI033993NIAID NIH HHS R01 AI112566NIAID NIH HHS R37 AI033993NIAID NIH HHS R38 AI140299NIAID NIH HHS U19 AI090023
6 · The paper itself

Abstract

Quantitative peptide-binding motifs of MHC class I alleles provide a valuable tool to efficiently identify putative T cell epitopes. Detailed information on equine MHC class I alleles is still very limited, and to date, only a single equine MHC class I allele, Eqca-1*00101 (ELA-A3 haplotype), has been characterized. The present study extends the number of characterized ELA class I specificities in two additional haplotypes found commonly in the Thoroughbred breed. Accordingly, we here report quantitative binding motifs for the ELA-A2 allele Eqca-16*00101 and the ELA-A9 allele Eqca-1*00201. Utilizing analyses of endogenously bound and eluted ligands and the screening of positional scanning combinatorial libraries, detailed and quantitative peptide-binding motifs were derived for both alleles. Eqca-16*00101 preferentially binds peptides with aliphatic/hydrophobic residues in position 2 and at the C-terminus, and Eqca-1*00201 has a preference for peptides with arginine in position 2 and hydrophobic/aliphatic residues at the C-terminus. Interestingly, the Eqca-16*00101 motif resembles that of the human HLA A02-supertype, while the Eqca-1*00201 motif resembles that of the HLA B27-supertype and two macaque class I alleles. It is expected that the identified motifs will facilitate the selection of candidate epitopes for the study of immune responses in horses.

Indexed as

Genes, MHC Class IAmino Acid MotifsAnimalsEpitopes, T-LymphocyteHaplotypesHistocompatibility Antigens Class IHorsesPeptide FragmentsProtein BindingProtein DomainsEpitopes, T-LymphocyteHistocompatibility Antigens Class IPeptide FragmentsEquus caballus (Eqca)MHC binding motifMHC class IPeptide bindingT cell epitope

Identifiers

PMID28315936
PMCPMC5555743
OpenAlexW2595983908

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.