ArticleJournal of virological methods2017
Absolute quantification of cassava brown streak virus mRNA by real-time qPCR.
Article in Journal of virological methods, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Electrochemical lateral flow assay with ELISA-level performance for detecting plant diseases in East Africa.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Epidemiological studies on the incidence of papaya ringspot disease under Indian sub-continent conditions.Scientific reports · 2025Article
- A robust absolute quantification protocol for potato virus Y based on reverse transcription-quantitative PCR.microPublication biology · 2025Article
- Occurrence and Distribution of Major Cassava Pests and Diseases in Cultivated Cassava Varieties in Western Kenya.Viruses · 2024Article
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- Differences in diacylglycerol acyltransferases expression patterns and regulation cause distinct hepatic triglyceride deposition in fish.Communications biology · 2024Article
- Quantitative detection of cassava common mosaic virus for health certification of cassava (Heliyon · 2024Article
- Genetic complexity of cassava brown streak disease: insights from qPCR-based viral titer analysis and genome-wide association studies.Frontiers in plant science · 2024Article
- Current knowledge and breeding strategies for management of aphid-transmitted viruses of pepper (Frontiers in plant science · 2024Review
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- TYMV and TRV infect Arabidopsis thaliana by expressing weak suppressors of RNA silencing and inducing host RNASE THREE LIKE1.PLoS pathogens · 2023Article
- A novel study on bean common mosaic virus accumulation shows disease resistance at the initial stage of infection inFrontiers in genetics · 2023Article
- Fast and Sensitive Detection of Soil-Borne Cereal Mosaic Virus in Leaf Crude Extract of Durum Wheat.Viruses · 2022Article
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- Insights into the effect of human civilization on Malus evolution and domestication.Plant biotechnology journal · 2021Article
- Indicators of the molecular pathogenesis of virulent Newcastle disease virus in chickens revealed by transcriptomic profiling of spleen.Scientific reports · 2021Article
- Establishment and characterization of the Bactrocera dorsalis (Diptera: Tephritidae) embryonic cell line QAU-Bd-E-2.In vitro cellular & developmental biology. Animal · 2021Article
- Outlook of Cassava Brown Streak Disease Assessment: Perspectives of the Screening Methods of Breeders and Pathologists.Frontiers in plant science · 2021Article
- Synergistic Effects of aFrontiers in plant science · 2021Article
- Absolute quantification of E. coli virulence and housekeeping genes to determine pathogen loads in enumerated environmental samples.PloS one · 2021Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cassava brown streak disease (CBSD) is the most important virus disease of cassava and a major food security threat in Africa. Yearly economic losses of up to $100 million USD have been attributed to CBSD. The lack of information on plant-virus interactions has restricted progress in breeding for CBSD resistance. Virus quantification is becoming a major tool for the quick and reliable assessment of plant host resistance. Therefore, a protocol for specific absolute quantification of Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV) was developed. CBSV and UCBSV coat protein (CP) specific standard templates: CBSV (pFer2, 826bp) and UCBSV (pUF1-R1-1, 732) respectively were generated and maintained in a TA cloning vector. These were used to construct standard curves using a TaqMan qPCR assay. Standard curves with acceptable amplification efficiencies (90-105%) and coefficients of determination (R
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