Trial reportJournal of the American Association for Laboratory Animal Science : JAALAS2017
Benefits of 21% Oxygen Compared with 100% Oxygen for Delivery of Isoflurane to Mice (
Trial report in Journal of the American Association for Laboratory Animal Science : JAALAS, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 30 citations in OpenAlex.
- Heterogeneity of Layer 1 Interneurons in the Mouse Medial Prefrontal Cortex.The Journal of comparative neurology · 2025Article
- An Overview of Anesthetic Procedures for Ferret (Mustela putorius furo) Preclinical Brain MRI: A Call for Standardization.Journal of the American Association for Laboratory Animal Science : JAALAS · 2025Review
- Anaesthesia-induced atelectasis in a rat model (Frontiers in veterinary science · 2025Article
- Cardiovascular function and autonomic regulation in urethane-anesthetized and conscious mice.American journal of physiology. Regulatory, integrative and comparative physiology · 2025Article
- Longitudinal alterations in brain perfusion and vascular reactivity in the zQ175DN mouse model of Huntington's disease.Journal of biomedical science · 2024Article
- Cardiopulmonary effects of apneustic anesthesia ventilation in anesthetized pigs: a new mode of ventilation for anesthetized veterinary species.Frontiers in veterinary science · 2024Article
- Volatile anaesthetic toxicity in the genetic mitochondrial disease Leigh syndrome.British journal of anaesthesia · 2023Article
- Protective Effect of Oxygen and Isoflurane in Rodent Model of Intestinal Ischemia-Reperfusion Injury.International journal of molecular sciences · 2023Article
- Mouse Anesthesia: The Art and Science.ILAR journal · 2021Review
- Effects of Oxygen Supplementation on Injectable and Inhalant Anesthesia in C57BL/6 Mice.Journal of the American Association for Laboratory Animal Science : JAALAS · 2021Article
- The (Un)Conscious Mouse as a Model for Human Brain Functions: Key Principles of Anesthesia and Their Impact on Translational Neuroimaging.Frontiers in systems neuroscience · 2020Review
- A genetic mouse model of severe iron deficiency anemia reveals tissue-specific transcriptional stress responses and cardiac remodeling.The Journal of biological chemistry · 2019Article
- Cardio-Respiratory synchronized bSSFP MRI for high throughput in vivo lung tumour quantification.PloS one · 2019Article
- Mapping regional strain in anesthetised healthy subjects during spontaneous ventilation.BMJ open respiratory research · 2019Article
- Ultrasound, photoacoustic, and magnetic resonance imaging to study hyperacute pathophysiology of traumatic and vascular brain injury.Journal of neuroimaging : official journal of the American Society of NeuroimagingArticle
Corrections and comments
- Erratum issuedErratum.2017
Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
At research institutions, isoflurane delivered by precision vaporizer to a face mask is the standard for rodent surgery and for procedures with durations that exceed a few minutes. Pure oxygen is often used as the carrier gas for isoflurane anesthesia, despite documented complications from long-term 100% oxygen use in humans and known occupational safety risks. We therefore examined the effect of anesthetic delivery gas on physiologic variables in mice and rats. Rodents were anesthetized for 60 min with isoflurane delivered in either 21% or 100% oxygen by means of a nose cone. We noted no difference between carrier gasses in physiologic variables in mice, including body temperature, respiratory rate, mean arterial pressure, surgical recovery time, pH, or PaCO2. However, blood gas analysis revealed evidence of a ventilation-perfusion mismatch in the 100% oxygen group. Pressure-volume hysteresis and histomorphometric analyses confirmed the presence of increased atelectasis in mice that received 100% oxygen. Unlike mice, rats that received isoflurane in 100% oxygen had acute respiratory acidosis and elevated mean arterial pressure, but atelectasis was similar between carrier gasses. Our data suggest that both 100% and 21% oxygen are acceptable for the delivery of isoflurane to mice. However, mice anesthetized for studies focused on lung physiology or architecture would benefit from the delivery of isoflurane in 21% oxygen to reduce absorption atelectasis and the potential associated downstream inflammatory effects. For rats, delivery of isoflurane in 21% and 100% oxygen both caused perturbations in physiologic variables, and choosing a carrier gas is not straightforward.
Indexed as
Identifiers
28315643PMC5361039W2627000462What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.