ArticleJournal of the American Society for Mass Spectrometry2017
Ammonium Bicarbonate Addition Improves the Detection of Proteins by Desorption Electrospray Ionization Mass Spectrometry.
Article in Journal of the American Society for Mass Spectrometry, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Immobilized Metal Affinity Beads for Recombinant Protein Analysis by Desorption Electrospray Ionization Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2026Article
- Accelerated oxidation of mustard gas analog with PVA-based hydrogels.RSC advances · 2025Article
- Nanobubbles in Electrospray Ionization Mass Spectrometry.Analytical chemistry · 2025Article
- Assessment of ammonium fluoride as a mobile phase additive for sensitivity gains in electrospray ionization.Analytical science advances · 2023Article
- Surface-sampling mass spectrometry to study proteins and protein complexes.Essays in biochemistry · 2023Review
- Approaches to Heterogeneity in Native Mass Spectrometry.Chemical reviews · 2022Review
- Mass Spectrometry Analysis of Intact Proteins from Crude Samples.Analytical chemistry · 2020Article
- Probing Protein Denaturation during Size-Exclusion Chromatography Using Native Mass Spectrometry.Analytical chemistry · 2020Article
- The Addition of Polar Organic Solvent Vapors During the Analysis of Proteins by DESI-MS.Journal of the American Society for Mass Spectrometry · 2019Article
- Addition of Serine Enhances Protein Analysis by DESI-MS.Journal of the American Society for Mass Spectrometry · 2019Article
- Enhanced Desorption Electrospray Ionization Mass Spectrometry via Synchronizing Ion Generation and Ion Injection.Journal of the American Society for Mass Spectrometry · 2019Article
- Optimised Desorption Electrospray Ionisation Mass Spectrometry Imaging (DESI-MSI) for the Analysis of Proteins/Peptides Directly from Tissue Sections on a Travelling Wave Ion Mobility Q-ToF.Journal of the American Society for Mass Spectrometry · 2018Article
- Comparing the Effects of Additives on Protein Analysis Between Desorption Electrospray (DESI) and Electrospray Ionization (ESI).Journal of the American Society for Mass Spectrometry · 2018Article
- Desorption Electrospray Ionization Mass Spectrometry Imaging of Proteins Directly from Biological Tissue Sections.Analytical chemistry · 2018Article
- Ambient ionisation mass spectrometry for in situ analysis of intact proteins.Journal of mass spectrometry : JMS · 2018Article
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Authors and funding
2 authors.
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Abstract
The analysis of protein by desorption electrospray ionization mass spectrometry (DESI-MS) is considered impractical due to a mass-dependent loss in sensitivity with increase in protein molecular weights. With the addition of ammonium bicarbonate to the DESI-MS analysis the sensitivity towards proteins by DESI was improved. The signal to noise ratio (S/N) improvement for a variety of proteins increased between 2- to 3-fold relative to solvent systems containing formic acid and more than seven times relative to aqueous methanol spray solvents. Three methods for ammonium bicarbonate addition during DESI-MS were investigated. The additive delivered improvements in S/N whether it was mixed with the analyte prior to sample deposition, applied over pre-prepared samples, or simply added to the desorption spray solvent. The improvement correlated well with protein pI but not with protein size. Other ammonium or bicarbonate salts did not produce similar improvements in S/N, nor was this improvement in S/N observed for ESI of the same samples. As was previously described for ESI, DESI also caused extensive protein unfolding upon the addition of ammonium bicarbonate. Graphical Abstract ᅟ.
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