ArticleRoux's archives of developmental biology : the official organ of the EDBO1991
In vitro culture ofDrosophila ovarian follicles: The influence of different media on development, RNA synthesis, protein synthesis and potassium uptake.
Article in Roux's archives of developmental biology : the official organ of the EDBO, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 22 citations in OpenAlex.
- Organ-resolved lipid mapping inFrontiers in chemistry · 2025Article
- Analysing bioelectrical phenomena in the Drosophila ovary with genetic tools: tissue-specific expression of sensors for membrane potential and intracellular pH, and RNAi-knockdown of mechanisms involved in ion exchange.BMC developmental biology · 2020Article
- Bioelectrical and cytoskeletal patterns correlate with altered axial polarity in the follicular epithelium of the Drosophila mutant gurken.BMC developmental biology · 2020Article
- Electrochemical gradients are involved in regulating cytoskeletal patterns during epithelial morphogenesis in the Drosophila ovary.BMC developmental biology · 2019Article
- Electrochemical patterns during Drosophila oogenesis: ion-transport mechanisms generate stage-specific gradients of pH and membrane potential in the follicle-cell epithelium.BMC developmental biology · 2019Article
- Relating proton pumps with gap junctions: colocalization of ductin, the channel-forming subunit c of V-ATPase, with subunit a and with innexins 2 and 3 during Drosophila oogenesis.BMC developmental biology · 2016Article
- Bioelectric patterning during oogenesis: stage-specific distribution of membrane potentials, intracellular pH and ion-transport mechanisms in Drosophila ovarian follicles.BMC developmental biology · 2015Article
- Gap junctions in the ovary of Drosophila melanogaster: localization of innexins 1, 2, 3 and 4 and evidence for intercellular communication via innexin-2 containing channels.BMC developmental biology · 2008Article
- Gap junctions in ovarian follicles of Drosophila melanogaster: inhibition and promotion of dye-coupling between oocyte and follicle cells.Cell and tissue research · 1993Article
- The specificity of yolk protein uptake in cyclorrhaphan diptera is conserved through evolution.Journal of molecular evolution · 1992Article
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Contradictory electrophysiological results were recently obtained inDrosophila ovarian follicles kept in different salines or complete media during measurements. Therefore, I checked follicles maintained in various solutions using morphological, physiological and biochemical criteria. Defined complete media were the best for supporting development from stage 10 to stage 14 (end of oogenesis). Supplementation of the solutions with insect pupal haemolymph had negative effects. For the maintenance of RNA synthesis, complete media were again superior to simple salines. Total protein synthesis was not very sensitive to the culture conditions during short incubation periods, but electrophoretic protein patterns were slightly less complex in the salines than in complete media. Furthermore, some major proteins (e.g. chorion proteins) synthesized during longterm culture failed to appear in the salines. In view of extrafollicular electrical currents and intracellular electrical potentials, potassium uptake experiments were conducted with several inhibitors, using rubidium-86 as a probe. Both potassium concentration and osmolarity were found to exert strong influences on total potassium uptake of the follicles. In the tested media and salines differing amounts of potassium were taken up via (Na
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