ArticleMolecular and cellular biology1987
Signal-dependent translocation of simian virus 40 large-T antigen into rat liver nuclei in a cell-free system.
Article in Molecular and cellular biology, 1987. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 48 citations in OpenAlex.
- Multiple regions of NSR1 are sufficient for accumulation of a fusion protein within the nucleolus.The Journal of cell biology · 1993Article
- Specific binding of nuclear localization sequences to plant nuclei.The Plant cell · 1993Article
- A cytoplasmically anchored nuclear protein interferes specifically with the import of nuclear proteins but not U1 snRNA.The Journal of cell biology · 1993Article
- A yeast protein that binds nuclear localization signals: purification localization, and antibody inhibition of binding activity.The Journal of cell biology · 1991Article
- Delineation of human papillomavirus type 18 enhancer binding proteins: the intracellular distribution of a novel octamer binding protein p92 is cell cycle regulated.Nucleic acids research · 1991Article
- The NSR1 gene encodes a protein that specifically binds nuclear localization sequences and has two RNA recognition motifs.The Journal of cell biology · 1991Article
- Karyophilic properties of Semliki Forest virus nucleocapsid protein.Journal of virology · 1990Article
- Nuclear protein import in permeabilized mammalian cells requires soluble cytoplasmic factors.The Journal of cell biology · 1990Article
- An N-ethylmaleimide-sensitive cytosolic factor necessary for nuclear protein import: requirement in signal-mediated binding to the nuclear pore.The Journal of cell biology · 1990Article
- Nuclear transport of proteins translated in vitro from SP6 plasmid-generated mRNAs.Molecular and cellular biology · 1990Article
- Identification and characterization of a nuclear localization sequence-binding protein in yeast.Proceedings of the National Academy of Sciences of the United States of America · 1989Article
- Yeast proteins that recognize nuclear localization sequences.The Journal of cell biology · 1989Article
- Identification of four nuclear transport signal-binding proteins that interact with diverse transport signals.Molecular and cellular biology · 1989Article
- Cell-dependent efficiency of reiterated nuclear signals in a mutant simian virus 40 oncoprotein targeted to the nucleus.Molecular and cellular biology · 1988Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
An in vitro nuclear translocation system is described in which isolated rat liver nuclei were incubated in a defined buffered medium containing radiolabeled or fluorescently labeled exogenous proteins. The nuclei were rapidly recovered, extracted, and analyzed for the presence of associated radiolabeled or fluorescently labeled proteins. The isolated nuclei exhibited the same specificity for protein uptake as seen previously in vivo, accumulating simian virus 40 wild-type large-T antigen and p53 while excluding a cytoplasmic variant of large-T antigen (d10) and bovine serum albumin. The rapid nuclear accumulation of wild-type large-T antigen was shown to be selective and dependent upon the recognition of a wild-type nuclear location signal, ATP and temperature dependent, and unidirectional. Taken together, the data suggest that in our in vitro system the nuclear translocation of wild-type large-T antigen exhibits some of the characteristics of an active transport process.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.