A Mouse Model for Human Unstable Hemoglobin Santa Ana.

Samantha I Miyashiro, Silvia M G Massironi, Claudia M C Mori, Carolina C Cruz, Mitika K Hagiwara, Paulo C Maiorka

Open access · greenAbstract read

In one paragraph

Article in Comparative medicine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact, top 70% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 3 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Samantha I MiyashiroMedical Clinic Institute of Biomedical Science, University of São Paulo, São Paulo, Brazil.

Silvia M G MassironiDepartment of Immunology, Institute of Biomedical Science, University of São Paulo, São Paulo, Brazil.

Claudia M C MoriDepartment of Pathology, School of Veterinary Medicine and AnimalMedicine and Animal Science Institute of Biomedical Science, University of São Paulo, São Paulo, Brazil;, Email: claumori@gmail.com.

Carolina C CruzDepartment of Immunology, Institute of Biomedical Science, University of São Paulo, São Paulo, Brazil.

Mitika K HagiwaraMedical Clinic Institute of Biomedical Science, University of São Paulo, São Paulo, Brazil.

Paulo C MaiorkaDepartment of Immunology, Institute of Biomedical Science, University of São Paulo, São Paulo, Brazil.

Universidade de São Paulo · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In the present study, we described the phenotype, histologic morphology, and molecular etiology of a mouse model of unstable hemoglobin Santa Ana. Hematologic evaluation of anemic mice (Anem/+) discovered after N-ethyl-N-nitrosourea mutagenesis revealed moderate anemia with intense reticulocytosis and polychromasia, followed by anisocytosis, macrocytosis, hypochromia, and intraerythrocytic inclusion and Heinz bodies. The mice also demonstrated hemoglobinuria, bilirubinemia, and erythrocytic populations with differing resistance to osmotic lysis. Splenomegaly (particularly in older mutant mice) and jaundice were apparent at necropsy. Histopathologic examination revealed dramatically increased hematopoiesis and hemosiderosis in hematopoietic organs and intracellular iron deposition in tubular renal cells. These data are characteristic of a congenital hemolytic regenerative anemia, similar to human anemias due to unstable hemoglobin. Genetic mapping assigned the affected gene to mouse chromosome 7, approximately 50 cM from the Hbb locus. The sequence of the mutant Hbb gene exhibited a T→C transversion at nucleotide 179 in Hbb-b1, leading to the substitution of proline for leucine at amino acid residue 88 and thus homologous to the genetic defect underlying Santa Ana anemia in humans.

Indexed as

Anemia, Hemolytic, CongenitalAnimalsChromosome MappingDisease Models, AnimalEthylnitrosoureaFemaleGenotypeHemoglobins, AbnormalHumansMaleMiceMice, Inbred BALB CMutationEthylnitrosoureaHemoglobins, Abnormalhemoglobin Santa Ana

Identifiers

PMID28304246

PMCPMC5157958

OpenAlexW2996335728

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.