ArticleScientific reports2017
Multiscale microenvironmental perturbation of pluripotent stem cell fate and self-organization.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- From genes to germ layers: virtual twins of gastruloids.NPJ systems biology and applications · 2026Review
- Organoid-based tissue engineering for advanced tissue repair and reconstruction.Materials today. Bio · 2025Review
- DNA microbeads for spatio-temporally controlled morphogen release within organoids.Nature nanotechnology · 2024Article
- Advancing Organoid Engineering for Tissue Regeneration and Biofunctional Reconstruction.Biomaterials research · 2024Review
- A Review of Advanced Biomaterials and Cells for the Production of Bone Organoid.Small science · 2023Article
- How might we build limbsFrontiers in cell and developmental biology · 2023Review
- Microfluidics for Neuronal Cell and Circuit Engineering.Chemical reviews · 2022Review
- Towards organoid culture without Matrigel.Communications biology · 2021Review
- Single-Cell Microgels for Diagnostics and Therapeutics.Advanced functional materials · 2021Article
- Bioinspired 3D Culture in Nanoliter Hyaluronic Acid-Rich Core-Shell Hydrogel Microcapsules Isolates Highly Pluripotent Human iPSCs.Small (Weinheim an der Bergstrasse, Germany) · 2021Article
- Alginate Microencapsulation for Three-Dimensional In Vitro Cell Culture.ACS biomaterials science & engineering · 2021Review
- A simple, low cost and reusable microfluidic gradient strategy and its application in modeling cancer invasion.Scientific reports · 2021Article
- Patterning Neuroepithelial Cell SheetACS biomaterials science & engineering · 2021Article
- Engineering organoids.Nature reviews. Materials · 2021Review
- Optogenetic Control of the BMP Signaling Pathway.ACS synthetic biology · 2020Article
- Engineering Heart Morphogenesis.Trends in biotechnology · 2020Review
- Engineered signaling centers for the spatially controlled patterning of human pluripotent stem cells.Nature methods · 2019Article
- Spatial presentation of biological molecules to cells by localized diffusive transfer.Lab on a chip · 2019Article
- User-defined morphogen patterning for directing human cell fate stratification.Scientific reports · 2019Article
- Progress and potential in organoid research.Nature reviews. Genetics · 2018Review
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The combination of microfluidics with engineered three-dimensional (3D) matrices can bring new insights into the fate regulation of stem cells and their self-organization into organoids. Although there has been progress in 3D stem cell culturing, most existing in vitro methodologies do not allow for mimicking of the spatiotemporal heterogeneity of stimuli that drive morphogenetic processes in vivo. To address this, we present a perfusion-free microchip concept for the in vitro 3D perturbation of stem cell fate. Stem cells are encapsulated in a hydrogel compartment that is flanked by open reservoirs for the diffusion-driven generation of biomolecule gradients. Juxtaposing additional compartments bearing supportive cells enables investigating the influence of long range cell-cell communication. We explore the utility of the microchips in manipulating early fate choices and self-organizing characteristics of 3D-cultured mouse embryonic stem cells (mESCs) under neural differentiation conditions and exposure to gradients of leukemia inhibitory factor (LIF). mESCs respond to LIF gradients in a spatially dependent manner. At higher LIF concentrations, multicellular colonies maintain pluripotency in contrast, at lower concentrations, mESCs develop into apicobasally polarized epithelial cysts. This versatile system can help to systematically explore the role of multifactorial microenvironments in promoting self-patterning of various stem cell types.
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Registered trials
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