ArticleMedical science monitor : international medical journal of experimental and clinical research2017
Effect of Hypoxia on Self-Renewal Capacity and Differentiation in Human Tendon-Derived Stem Cells.
Article in Medical science monitor : international medical journal of experimental and clinical research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Shared mechanisms of musculoskeletal dysfunction in type 2 diabetes mellitus: Insights into future therapeutic directions (Review).International journal of molecular medicine · 2026Review
- Hypoxia-Inducible Factor and Oxidative Stress in Tendon Degeneration: A Molecular Perspective.Antioxidants (Basel, Switzerland) · 2024Review
- Role of tendon-derived stem cells in tendon and ligament repair: focus on tissue engineer.Frontiers in bioengineering and biotechnology · 2024Review
- Effect of Aging on Tendon Biology, Biomechanics and Implications for Treatment Approaches.International journal of molecular sciences · 2023Review
- N-Acetyl-L-cysteine facilitates tendon repair and promotes the tenogenic differentiation of tendon stem/progenitor cells by enhancing the integrin α5/β1/PI3K/AKT signaling.BMC molecular and cell biology · 2023Article
- Physiologic isolation and expansion of human mesenchymal stem/stromal cells for manufacturing of cell-based therapy products.Engineering in life sciences · 2022Review
- [Research progress of interfacial tissue engineering in rotator cuff repair].Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery · 2021Article
- Characterization of Tendon-Derived Stem Cells and Rescue Tendon Injury.Stem cell reviews and reports · 2021Review
- Insight into Hypoxia Stemness Control.Cells · 2021Review
- Kinship of conditionally immortalized cells derived from fetal bone to human bone-derived mesenchymal stroma cells.Scientific reports · 2021Article
- Metabolic Regulation of Tendon Inflammation and Healing Following Injury.Current rheumatology reports · 2021Review
- Stem Cells for the Regeneration of Tendon and Ligament: A Perspective.International journal of stem cells · 2020Review
- In Vitro Innovation of Tendon Tissue Engineering Strategies.International journal of molecular sciences · 2020Review
- Hypoxia-Induced Mesenchymal Stem Cells Exhibit Stronger Tenogenic Differentiation Capacities and Promote Patellar Tendon Repair in Rabbits.Stem cells international · 2020Article
- The Effects of Hypoxia-Reoxygenation in Mouse Digital Flexor Tendon-Derived Cells.Oxidative medicine and cellular longevity · 2020Article
- Tendon stem progenitor cells: Understanding the biology to inform therapeutic strategies for tendon repair.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2019Review
- Chemical Activation of the Hypoxia-Inducible Factor Reversibly Reduces Tendon Stem Cell Proliferation, Inhibits Their Differentiation, and Maintains Cell Undifferentiation.Stem cells international · 2018Article
- Transcriptomic Profiling Reveals NF-κB-Associated Immune Regulatory Signatures Underlying the Regenerative Effects of Hypoxia-Preconditioned Tendon Stem Cell-Derived Extracellular Vesicles.BioFactors (Oxford, England)Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND Hypoxic conditions play roles in functioning of human tendon-derived stem cells (hTSCs). The goal of this study was to investigate the effect of various hypoxic conditions in self-renewal capacity and differentiation of TSCs. MATERIAL AND METHODS hTSCs was obtain from supraspinatus tendon donors. Colony formation and cell proliferation assay were used to assess the self-renewal of hTSCs. qRT-PCT and Western blot analysis were used to examine stemness and multi-differentiation potential of hTSCs. RESULTS We found that culturing at 5% O2 is more beneficial for the self-renewal of hTSCs than the other 3 culture conditions, with larger colony size and numbers. The proliferation of hTSCs in 5%, 10%, and 20% O2 cultures increased after seeding. The number of cells in the 5% O2 condition was higher than that in other culture; however, self-renewal capacity of hTSCs in 0.5% O2 was inhibited. The expression levels of stem cell markers, including NS, Nanog, Oct-4, and SSEA-4, were highest in 0.5% O2 culture. Furthermore, hTSCs cultured in 20% O2 exhibited significantly higher expression of the 3 markers (PPAR-γ, Sox-9, and Runx-2). CONCLUSIONS Hypoxic condition of culture encouraged self-renewal capacity of hTSCs, but inhibited their multi-differentiation potential, compared to normoxic condition of culture. Moreover, excessively low oxygen concentration impaired the capacity of hTSCs.
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