ArticleScience (New York, N.Y.)2017
Structures of the cyanobacterial circadian oscillator frozen in a fully assembled state.
Article in Science (New York, N.Y.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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Who cites it
64 citing papers in PubMed.
- Review
- Reconstitution of circadian clock in synthetic cells reveals principles of timekeeping.Nature communications · 2025Article
- X-ray spectroscopy meets native mass spectrometry: probing gas-phase protein complexes.Physical chemistry chemical physics : PCCP · 2025Review
- Programming scheduled self-assembly of circadian materials.Nature communications · 2025Article
- The cyanobacterial circadian clock.Npj biological timing and sleep · 2025Review
- KaiC family ATPases in the nonheterocystous nitrogen-fixing cyanobacterium Leptolyngbya boryana.Scientific reports · 2024Article
- Temperature-dependent fold-switching mechanism of the circadian clock protein KaiB.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- A topological mechanism for robust and efficient global oscillations in biological networks.Nature communications · 2024Article
- Article
- Article
- The inner workings of an ancient biological clock.Trends in biochemical sciences · 2024Review
- Cryo-EM of soft-landed β-galactosidase: Gas-phase and native structures are remarkably similar.Science advances · 2024Article
- Structure-function relationship of KaiC around dawn.Biophysics and physicobiology · 2024Article
- Determining subunit-subunit interaction from statistics of cryo-EM images: observation of nearest-neighbor coupling in a circadian clock protein complex.Nature communications · 2023Article
- Dissecting the structural heterogeneity of proteins by native mass spectrometry.Protein science : a publication of the Protein Society · 2023Review
- The increasing role of structural proteomics in cyanobacteria.Essays in biochemistry · 2023Review
- Cryo-EM samples of gas-phase purified protein assemblies using native electrospray ion-beam deposition.Faraday discussions · 2022Article
- Mass-selective and ice-free electron cryomicroscopy protein sample preparation via native electrospray ion-beam deposition.PNAS nexus · 2022Article
- Role of the reaction-structure coupling in temperature compensation of the KaiABC circadian rhythm.PLoS computational biology · 2022Article
- Coupling of distant ATPase domains in the circadian clock protein KaiC.Nature structural & molecular biology · 2022Article
4 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cyanobacteria have a robust circadian oscillator, known as the Kai system. Reconstituted from the purified protein components KaiC, KaiB, and KaiA, it can tick autonomously in the presence of adenosine 5'-triphosphate (ATP). The KaiC hexamers enter a natural 24-hour reaction cycle of autophosphorylation and assembly with KaiB and KaiA in numerous diverse forms. We describe the preparation of stoichiometrically well-defined assemblies of KaiCB and KaiCBA, as monitored by native mass spectrometry, allowing for a structural characterization by single-particle cryo-electron microscopy and mass spectrometry. Our data reveal details of the interactions between the Kai proteins and provide a structural basis to understand periodic assembly of the protein oscillator.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.