ArticleThe Journal of general physiology2017
Bright flash response recovery of mammalian rods in vivo is rate limited by RGS9.
Article in The Journal of general physiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 20 citations in OpenAlex.
- RGS proteins: Potential regulators of mouse melanopsin's signaling phototransduction cascade across ipRGC subtypes.Biophysical journal · 2026Article
- Temporal Sensitivity Under Photopic and Scotopic Conditions Across the Cortical Visual Hierarchy.Investigative ophthalmology & visual science · 2025Article
- Temporal Transformation of the Rod Single-Photon Response in the Retinal Circuitry.Investigative ophthalmology & visual science · 2025Article
- The Role of Individual Residues in the N-Terminus of Arrestin-1 in Rhodopsin Binding.International journal of molecular sciences · 2025Article
- Diversity and Evolution of Frog Visual Opsins: Spectral Tuning and Adaptation to Distinct Light Environments.Molecular biology and evolution · 2024Article
- A Simplified Model of Activation and Deactivation of Human Rod Phototransduction-An Electroretinographic Study.Investigative ophthalmology & visual science · 2023Article
- Photoreceptor phosphodiesterase (PDE6): activation and inactivation mechanisms during visual transduction in rods and cones.Pflugers Archiv : European journal of physiology · 2021Review
- Functional optical coherence tomography enablesBiomedical optics express · 2020Article
- Rod Photoresponse Kinetics Limit Temporal Contrast Sensitivity in Mesopic Vision.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- Loss of cone function without degeneration in a novel Gnat2 knock-out mouse.Experimental eye research · 2018Article
- The discovery of the ability of rod photoreceptors to signal single photons.The Journal of general physiology · 2018Review
- RBX2 maintains final retinal cell position in a DAB1-dependent and -independent fashion.Development (Cambridge, England) · 2018Article
- In vivo optophysiology reveals that G-protein activation triggers osmotic swelling and increased light scattering of rod photoreceptors.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The temporal resolution of scotopic vision is thought to be constrained by the signaling kinetics of retinal rods, which use a highly amplified G-protein cascade to transduce absorbed photons into changes in membrane potential. Much is known about the biochemical mechanisms that determine the kinetics of rod responses ex vivo, but the rate-limiting mechanisms in vivo are unknown. Using paired flash electroretinograms with improved signal-to-noise, we have recorded the amplitude and kinetics of rod responses to a wide range of flash strengths from living mice. Bright rod responses in vivo recovered nearly twice as fast as all previous recordings, although the kinetic consequences of genetic perturbations previously studied ex vivo were qualitatively similar. In vivo, the dominant time constant of recovery from bright flashes was dramatically reduced by overexpression of the RGS9 complex, revealing G-protein deactivation to be rate limiting for recovery. However, unlike previous ex vivo recordings, dim flash responses in vivo were relatively unaffected by RGS9 overexpression, suggesting that other mechanisms, such as calcium feedback dynamics that are strongly regulated by the restricted subretinal microenvironment, act to determine rod dim flash kinetics. To assess the consequences for scotopic vision, we used a nocturnal wheel-running assay to measure the ability of wild-type and RGS9-overexpressing mice to detect dim flickering stimuli and found no improvement when rod recovery was speeded by RGS9 overexpression. These results are important for understanding retinal circuitry, in particular as modeled in the large literature that addresses the relationship between the kinetics and sensitivity of retinal responses and visual perception.
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