Evidence map›Paper›PMID 28302649›Full record

ArticleCirculation research2017

Endothelial Protective Monocyte Patrolling in Large Arteries Intensified by Western Diet and Atherosclerosis.

Amado Quintar, Sara McArdle, Dennis Wolf, Alex Marki, Erik Ehinger, Melanie Vassallo, Jacqueline Miller, Zbigniew Mikulski, Klaus Ley, Konrad Buscher

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Circulation research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
70citing papers in PubMed, 2 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

70 citing papers in PubMed, 2 syntheses or guidelines pooled it.

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10 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors.

Amado QuintarFrom the La Jolla Institute for Allergy and Immunology, Division of Inflammation Biology, La Jolla, CA (A.Q., S.M., D.W., A.M., E.E., M.V., J.M., Z.M., K.L., K.B.); and Centro de Microscopia Electronica, INICSA-CONICET, Facultad de Ciencias Medicas, Universidad Nacional de Cordoba, Cordoba, Argentina (A.Q.).
Sara McArdleFrom the La Jolla Institute for Allergy and Immunology, Division of Inflammation Biology, La Jolla, CA (A.Q., S.M., D.W., A.M., E.E., M.V., J.M., Z.M., K.L., K.B.); and Centro de Microscopia Electronica, INICSA-CONICET, Facultad de Ciencias Medicas, Universidad Nacional de Cordoba, Cordoba, Argentina (A.Q.).
Dennis WolfFrom the La Jolla Institute for Allergy and Immunology, Division of Inflammation Biology, La Jolla, CA (A.Q., S.M., D.W., A.M., E.E., M.V., J.M., Z.M., K.L., K.B.); and Centro de Microscopia Electronica, INICSA-CONICET, Facultad de Ciencias Medicas, Universidad Nacional de Cordoba, Cordoba, Argentina (A.Q.).
Alex MarkiFrom the La Jolla Institute for Allergy and Immunology, Division of Inflammation Biology, La Jolla, CA (A.Q., S.M., D.W., A.M., E.E., M.V., J.M., Z.M., K.L., K.B.); and Centro de Microscopia Electronica, INICSA-CONICET, Facultad de Ciencias Medicas, Universidad Nacional de Cordoba, Cordoba, Argentina (A.Q.).
Erik EhingerFrom the La Jolla Institute for Allergy and Immunology, Division of Inflammation Biology, La Jolla, CA (A.Q., S.M., D.W., A.M., E.E., M.V., J.M., Z.M., K.L., K.B.); and Centro de Microscopia Electronica, INICSA-CONICET, Facultad de Ciencias Medicas, Universidad Nacional de Cordoba, Cordoba, Argentina (A.Q.).
Melanie VassalloFrom the La Jolla Institute for Allergy and Immunology, Division of Inflammation Biology, La Jolla, CA (A.Q., S.M., D.W., A.M., E.E., M.V., J.M., Z.M., K.L., K.B.); and Centro de Microscopia Electronica, INICSA-CONICET, Facultad de Ciencias Medicas, Universidad Nacional de Cordoba, Cordoba, Argentina (A.Q.).
Jacqueline MillerFrom the La Jolla Institute for Allergy and Immunology, Division of Inflammation Biology, La Jolla, CA (A.Q., S.M., D.W., A.M., E.E., M.V., J.M., Z.M., K.L., K.B.); and Centro de Microscopia Electronica, INICSA-CONICET, Facultad de Ciencias Medicas, Universidad Nacional de Cordoba, Cordoba, Argentina (A.Q.).
Zbigniew MikulskiFrom the La Jolla Institute for Allergy and Immunology, Division of Inflammation Biology, La Jolla, CA (A.Q., S.M., D.W., A.M., E.E., M.V., J.M., Z.M., K.L., K.B.); and Centro de Microscopia Electronica, INICSA-CONICET, Facultad de Ciencias Medicas, Universidad Nacional de Cordoba, Cordoba, Argentina (A.Q.).
Klaus LeyFrom the La Jolla Institute for Allergy and Immunology, Division of Inflammation Biology, La Jolla, CA (A.Q., S.M., D.W., A.M., E.E., M.V., J.M., Z.M., K.L., K.B.); and Centro de Microscopia Electronica, INICSA-CONICET, Facultad de Ciencias Medicas, Universidad Nacional de Cordoba, Cordoba, Argentina (A.Q.).
Konrad BuscherFrom the La Jolla Institute for Allergy and Immunology, Division of Inflammation Biology, La Jolla, CA (A.Q., S.M., D.W., A.M., E.E., M.V., J.M., Z.M., K.L., K.B.); and Centro de Microscopia Electronica, INICSA-CONICET, Facultad de Ciencias Medicas, Universidad Nacional de Cordoba, Cordoba, Argentina (A.Q.). konrad@lji.org klaus@lji.org.

Funding

Integrative Bioengineering of Heart, Vessels, and BloodT32HL105373 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI CHIEN, SHU, INTAGLIETTA, MARCOS · 2010 to 2019
$3.6M
Myeloid cell interactions with T cells in atherosclerosisR01HL115232 · NHLBI · LA JOLLA INSTITUTE FOR IMMUNOLOGY · PI LEY, KLAUS F. · 2012 to 2018
$3.2M
NHLBI NIH HHS R01 HL115232NHLBI NIH HHS T32 HL105373
6 · The paper itself

Abstract

rationaleNonclassical mouse monocyte (CX3CR1

objectiveThis study was undertaken to elucidate the molecular, migratory, and functional phenotypes of patrolling monocytes in the high shear and pulsatile environment of large arteries in healthy, hyperlipidemic, and atherosclerotic conditions. METHODS AND

resultsApplying a new method for stable, long-term 2-photon intravital microscopy of unrestrained large arteries in live CX3CR1-GFP (green fluorescent protein) mice, we show that nonclassical monocytes patrol inside healthy carotid arteries at a velocity of 36 μm/min, 3× faster than in microvessels. The tracks are less straight but lead preferentially downstream. The number of patrolling monocytes is increased 9-fold by feeding wild-type mice a Western diet or by applying topical TLR7/8 (Toll-like receptor) agonists. A similar increase is seen in CX3CR1

conclusionsArterial patrolling is a prominent new feature of nonclassical monocytes with unique molecular and kinetic properties. It is highly upregulated in hyperlipidemia and atherosclerosis in a CX3CR1-independent fashion and plays a potential role in endothelial protection.

Indexed as

AnimalsArteriesAtherosclerosisCX3C Chemokine Receptor 1Diet, WesternEndothelium, VascularMiceMice, Inbred C57BLMice, KnockoutMonocytesReceptors, ChemokineCX3C Chemokine Receptor 1Cx3cr1 protein, mouseReceptors, Chemokineapoptosisatherosclerosisendotheliumhomeostasisinflammation

Identifiers

PMID28302649
PMCPMC5446289

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.