ArticlePloS one2017
Dissection of TALE-dependent gene activation reveals that they induce transcription cooperatively and in both orientations.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 28 citations in OpenAlex.
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Proteomic analysis reveals dynamic expression related to malondialdehyde in cassava in response to cassava bacterial blight.Scientific reports · 2025Article
- A promoter trap in transgenic citrus mediates recognition of a broad spectrum of Xanthomonas citri pv. citri TALEs, including in planta-evolved derivatives.Plant biotechnology journal · 2023Article
- Assembling highly repetitive Xanthomonas TALomes using Oxford Nanopore sequencing.BMC genomics · 2023Article
- A single promoter-TALE system for tissue-specific and tuneable expression of multiple genes in rice.Plant biotechnology journal · 2022Article
- The RiceInternational journal of molecular sciences · 2022Article
- An ERF121 transcription factor from Brassica oleracea is a target for the conserved TAL-effectors from different Xanthomonas campestris pv. campestris strains.Molecular plant pathology · 2021Article
- Complete Genome Assemblies of AllFrontiers in microbiology · 2021Article
- The genetic arms race between plant and Xanthomonas: lessons learned from TALE biology.Science China. Life sciences · 2021Review
- TALEN-based editing of TFIIAy5 changes rice response to Xanthomonas oryzae pv. Oryzae.Scientific reports · 2020Article
- PthA4Molecular plant pathology · 2019Article
- PrediTALE: A novel model learned from quantitative data allows for new perspectives on TALE targeting.PLoS computational biology · 2019Article
- Transcriptional Reprogramming of Rice Cells byFrontiers in plant science · 2019Article
- Correction: Dissection of TALE-dependent gene activation reveals that they induce transcription cooperatively and in both orientations.PloS one · 2017Article
Corrections and comments
- Erratum issuedCorrection: Dissection of TALE-dependent gene activation reveals that they induce transcription cooperatively and in both orientations.2017 · Stuttman J [corrected to Stuttmann J]
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant-pathogenic Xanthomonas bacteria inject transcription activator-like effector proteins (TALEs) into host cells to specifically induce transcription of plant genes and enhance susceptibility. Although the DNA-binding mode is well-understood it is still ambiguous how TALEs initiate transcription and whether additional promoter elements are needed to support this. To systematically dissect prerequisites for transcriptional initiation the activity of one TALE was compared on different synthetic Bs4 promoter fragments. In addition, a large collection of artificial TALEs spanning the OsSWEET14 promoter was compared. We show that the presence of a TALE alone is not sufficient to initiate transcription suggesting the requirement of additional supporting promoter elements. At the OsSWEET14 promoter TALEs can initiate transcription from various positions, in a synergistic manner of multiple TALEs binding in parallel to the promoter, and even by binding in reverse orientation. TALEs are known to shift the transcriptional start site, but our data show that this shift depends on the individual position of a TALE within a promoter context. Our results implicate that TALEs function like classical enhancer-binding proteins and initiate transcription in both orientations which has consequences for in planta target gene prediction and design of artificial activators.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.