ArticleNature protocols2017
Dissection of mechanical force in living cells by super-resolved traction force microscopy.
Article in Nature protocols, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
43 citing papers in PubMed, 73 citations in OpenAlex.
- Optical Detection of Cellular Signals at Material Interfaces.Advanced healthcare materials · 2026Review
- 3D traction force microscopy in human trabecular meshwork tissues: Effects of ROCK and YAP/TAZ inhibition in normal and glaucomatous tissues.Tissue & cell · 2025Article
- Microneedle-Based DNA Tension Gauge Tethers Enable In Vivo Monitoring of Cell Mechanics during Skin Tissue Regeneration.JACS Au · 2025Article
- Vascular smooth muscle cells can be circumferentially aligned inside a channel using tunable gelatin microribbons.Biofabrication · 2024Article
- Scar matrix drives Piezo1 mediated stromal inflammation leading to placenta accreta spectrum.Nature communications · 2024Article
- Real-time monitoring of intracellular biochemical response in locally stretched single cell by a nanosensor.Analytical and bioanalytical chemistry · 2024Article
- Stable and oscillatory hypoxia differentially regulate invasibility of breast cancer associated fibroblasts.Mechanobiology in medicine · 2024Article
- Mechanical control of antigen detection and discrimination by T and B cell receptors.Biophysical journal · 2024Review
- Multimodal analysis of traction forces and the temperature dynamics of living cells with a diamond-embedded substrate.Biomedical optics express · 2024Article
- Molecular Force Sensors for Biological Application.International journal of molecular sciences · 2024Review
- Comparative analysis of traction forces in normal and glaucomatous trabecular meshwork cells within a 3D, active fluid-structure interaction culture environment.Acta biomaterialia · 2024Article
- Field Guide to Traction Force Microscopy.Cellular and molecular bioengineering · 2024Review
- Dynamic traction force in trabecular meshwork cells: A 2D culture model for normal and glaucomatous states.Acta biomaterialia · 2024Article
- Hydrogel-based molecular tension fluorescence microscopy for investigating receptor-mediated rigidity sensing.Nature methods · 2023Article
- Trends in mechanobiology guided tissue engineering and tools to study cell-substrate interactions: a brief review.Biomaterials research · 2023Review
- Deep-learning-based 3D cellular force reconstruction directly from volumetric images.Biophysical journal · 2022Article
- A vasculature niche orchestrates stromal cell phenotype through PDGF signaling: Importance in human fibrotic disease.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Stiff matrices enhance myoblast proliferation, reduce differentiation, and alter the response to fluid shear stress in vitro.Cell biochemistry and biophysics · 2022Article
- Cells on Hydrogels with Micron-Scaled Stiffness Patterns Demonstrate Local Stiffness Sensing.Nanomaterials (Basel, Switzerland) · 2022Article
- May the force be with your (immune) cells: an introduction to traction force microscopy in Immunology.Frontiers in immunology · 2022Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Cells continuously exert or respond to mechanical force. Measurement of these nanoscale forces is a major challenge in cell biology; yet such measurement is essential to the understanding of cell regulation and function. Current methods for examining mechanical force generation either necessitate dedicated equipment or limit themselves to coarse-grained force measurements on the micron scale. In this protocol, we describe stimulated emission depletion traction force microscopy-STED-TFM (STFM), which allows higher sampling of the forces generated by the cell than conventional TFM, leading to a twofold increase in spatial resolution (of up to 500 nm). The procedure involves the preparation of functionalized polyacrylamide gels loaded with fluorescent beads, as well as the acquisition of STED images and their analysis. We illustrate the approach using the example of HeLa cells expressing paxillin-EGFP to visualize focal adhesions. Our protocol uses widely available laser-scanning confocal microscopes equipped with a conventional STED laser, open-source software and common molecular biology techniques. The entire STFM experiment preparation, data acquisition and analysis require 2-3 d and could be completed by someone with minimal experience in molecular biology or biophysics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.