ArticleScientific reports2017
Brucella spp. of amphibians comprise genomically diverse motile strains competent for replication in macrophages and survival in mammalian hosts.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers, 1 of them a synthesis that pooled it.
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Who cites it
48 citing papers in PubMed, 1 synthesis or guideline pooled it, 88 citations in OpenAlex.
- A Systematic Review and Meta-Analysis on the Prevalence of Brucella melitensis in Sheep and Goat.Veterinary medicine and science · 2025Pooled it
- Two novel real-time PCR assays forMicrobiology spectrum · 2026Article
- Vegetation composition shapes denitrifier community structure and enhances nitrogen removal in estuarine wetlands: evidence from Reed-Willow Mix promotingCurrent research in microbial sciences · 2026Article
- The atypical quorum sensing system of classicalJournal of bacteriology · 2025Review
- A new member of the novel, non-core Brucella clade: An exotic frog isolate closely related to atypical Brucella isolates from recent human brucellosis cases in Australia.BMC microbiology · 2025Article
- Investigation of genomic island 2 deciphers the evolution of a genus Brucella.Scientific reports · 2025Article
- Role of RB51 vaccine in cow milk contamination of traditional milk sales centers.Tropical animal health and production · 2025Article
- Brucella microti and Rodent-Borne Brucellosis: A Neglected Public Health Threat.Zoonoses and public health · 2025Review
- Brucellosis: Bacteriology, pathogenesis, epidemiology and role of the metallophores in virulence: a review.Frontiers in cellular and infection microbiology · 2025Review
- Article
- Molecular typing methods to characterizeVeterinary world · 2024Review
- TheAnnual review of microbiology · 2023Review
- Cell and Tissue Tropism of Brucella spp.Infection and immunity · 2023Review
- Oral vaccination with novel Lactococcus lactis mucosal live vector-secreting Brucella lumazine synthase (BLS) protein induces humoral and cellular immune protection against Brucella abortus.Archives of microbiology · 2023Article
- Isolation ofFrontiers in microbiology · 2023Article
- Article
- Core Genome Multilocus Sequence Typing Scheme for Improved Characterization and Epidemiological Surveillance of Pathogenic Brucella.Journal of clinical microbiology · 2022Article
- The Retrospective on AtypicalMicroorganisms · 2022Review
- Review
- Atypical Brucella inopinata-Like Species in 2 Marine Toads.Emerging infectious diseases · 2021Article
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Authors and funding
15 authors at 13 institutions in 5 countries.
Funding
Abstract
Twenty-one small Gram-negative motile coccobacilli were isolated from 15 systemically diseased African bullfrogs (Pyxicephalus edulis), and were initially identified as Ochrobactrum anthropi by standard microbiological identification systems. Phylogenetic reconstructions using combined molecular analyses and comparative whole genome analysis of the most diverse of the bullfrog strains verified affiliation with the genus Brucella and placed the isolates in a cluster containing B. inopinata and the other non-classical Brucella species but also revealed significant genetic differences within the group. Four representative but molecularly and phenotypically diverse strains were used for in vitro and in vivo infection experiments. All readily multiplied in macrophage-like murine J774-cells, and their overall intramacrophagic growth rate was comparable to that of B. inopinata BO1 and slightly higher than that of B. microti CCM 4915. In the BALB/c murine model of infection these strains replicated in both spleen and liver, but were less efficient than B. suis 1330. Some strains survived in the mammalian host for up to 12 weeks. The heterogeneity of these novel strains hampers a single species description but their phenotypic and genetic features suggest that they represent an evolutionary link between a soil-associated ancestor and the mammalian host-adapted pathogenic Brucella species.
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