ArticleScientific reports2017
Evaluation of reference genes at different developmental stages for quantitative real-time PCR in Aedes aegypti.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.
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Who cites it
69 citing papers in PubMed, 124 citations in OpenAlex.
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- Tolerance to persistent cell-fusing agent virus infection in Aedes aegypti mosquitoes correlates with maintenance of lipid reserves.BMC biology · 2026Article
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- Selection of Stable Reference Genes for Gene Expression Studies in Activated and Non-Activated PBMCs Under Normoxic and Hypoxic Conditions.International journal of molecular sciences · 2025Article
- Evaluation of stable reference genes for RT-qPCR analysis in Antheraea assamensis (Lepidoptera: Saturniidae) during pathogenic infections.Molecular biology reports · 2025Article
- Characterization of the transcriptional cellular response in midgut tissue of temephos-resistant Aedes aegypti larvae.Parasites & vectors · 2025Article
- Validation of reliable reference genes for comparison of gene expression across species in the Anopheles Hyrcanus Group.Scientific reports · 2025Article
- One-Step Multiplex Polymerase Chain Reaction Assay for the Detection of Major Disease-Transmitting Mosquito Vectors in India.The American journal of tropical medicine and hygiene · 2025Article
- A single-cell atlas of the Culex tarsalis midgut during West Nile virus infection.PLoS pathogens · 2025Article
- A single-cell atlas of thebioRxiv : the preprint server for biology · 2024Article
- Sequencing RNA from old, dried specimens reveals past viromes and properties of long-surviving RNA.bioRxiv : the preprint server for biology · 2024Article
- Stability and suitability of housekeeping genes in phlebotomine sand flies.Scientific reports · 2024Article
- Selection and Validation of Reference Genes for Quantitative Real-Time PCR Analysis in Cockroach ParasitoidInsects · 2024Article
9 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mosquito Aedes aegypti (Ae. aegypti) is the most notorious vector of illness-causing viruses such as Dengue, Chikugunya, and Zika. Although numerous genetic expression studies utilizing quantitative real-time PCR (qPCR) have been conducted with regards to Ae. aegypti, a panel of genes to be used suitably as references for the purpose of expression-level normalization within this epidemiologically important insect is presently lacking. Here, the usability of seven widely-utilized reference genes i.e. actin (ACT), eukaryotic elongation factor 1 alpha (eEF1α), alpha tubulin (α-tubulin), ribosomal proteins L8, L32 and S17 (RPL8, RPL32 and RPS17), and glyceraldeyde 3-phosphate dehydrogenase (GAPDH) were investigated. Expression patterns of the reference genes were observed in sixteen pre-determined developmental stages and in cell culture. Gene stability was inferred from qPCR data through three freely available algorithms i.e. BestKeeper, geNorm, and NormFinder. The consensus rankings generated from stability values provided by these programs suggest a combination of at least two genes for normalization. ACT and RPS17 are the most dependably expressed reference genes and therefore, we propose an ACT/RPS17 combination for normalization in all Ae. aegypti derived samples. GAPDH performed least desirably, and is thus not a recommended reference gene. This study emphasizes the importance of validating reference genes in Ae. aegypti for qPCR based research.
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