Evidence map›Paper›PMID 28299740›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2017

Detection of Matrix Metalloproteinases by Zymography.

Rajeev B Tajhya, Rutvik S Patel, Christine Beeton

Open access · greenAbstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed
15.6field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed, 63 citations in OpenAlex.

  1. Trial
  2. Article
  3. Article
  4. Gelatin Zymography: Principle, Method and Application in Cancer Research.Methods in molecular biology (Clifton, N.J.) · 2025
    Article
  5. Analysis of Metalloproteinase Secretion in Cancer Cells by Zymography.Methods in molecular biology (Clifton, N.J.) · 2025
    Article
  6. Article
  7. Article
  8. Article
  9. Research in pharmaceutical sciences · 2024
    Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Review
  16. Article
  17. Article
  18. Article
  19. In Vitro Models to Study Angiogenesis and Vasculature.Methods in molecular biology (Clifton, N.J.) · 2022
    Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Rajeev B TajhyaDepartment of Molecular Physiology and Biophysics, Baylor College of Medicine, One Baylor Plaza, Houston, TX, 77030, USA.
Rutvik S PatelDepartment of Molecular Physiology and Biophysics, Baylor College of Medicine, One Baylor Plaza, Houston, TX, 77030, USA.
Christine BeetonDepartment of Molecular Physiology and Biophysics, Baylor College of Medicine, One Baylor Plaza, Houston, TX, 77030, USA. beeton@bcm.edu.
Baylor College of Medicine · US

Funding

New Peptides for the Treatment of Multiple SclerosisR01NS073712 · NINDS · BAYLOR COLLEGE OF MEDICINE · PI BEETON, CHRISTINE · 2011 to 2015
$1.8M
Potassium channels in myotonic dystrophy type 1R03AR059838 · NIAMS · BAYLOR COLLEGE OF MEDICINE · PI BEETON, CHRISTINE · 2010 to 2012
$224k
NIAMS NIH HHS R03 AR059838NINDS NIH HHS R01 NS073712
6 · The paper itself

Abstract

Matrix metalloproteinases (MMPs) represent more than 20 zinc-containing endopeptidases that cleave internal peptide bonds, leading to protein degradation. They play a critical role in many physiological cell functions, including tissue remodeling, embryogenesis, and angiogenesis. They are also involved in the pathogenesis of a vast array of diseases, including but not limited to systemic inflammation, various cancers, and cardiovascular, neurological, and autoimmune diseases. Here, we describe gel zymography to detect MMPs in cell and tissue samples and in cell culture supernatants.

Indexed as

AnimalsCells, CulturedElectrophoresis, Polyacrylamide GelHumansMatrix MetalloproteinasesMatrix MetalloproteinasesDetectionProteaseSemiquantitativeZymogramZymography

Identifiers

PMID28299740
PMCPMC5465868
OpenAlexW2680992019

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.