ArticleMolecular biology of the cell2017
Mechanisms of kinetic stabilization by the drugs paclitaxel and vinblastine.
Article in Molecular biology of the cell, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 70 citations in OpenAlex.
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- Role of α-tubulin helix 11' in heterodimer conformation and microtubule dynamics.bioRxiv : the preprint server for biology · 2026Article
- Oscillatory dynamics in paclitaxel-proteinoid networks.Discover molecules · 2026Article
- Drugs from poisonous plants: Ethnopharmacological relevance to modern perspectives.Toxicon: X · 2025Review
- Precise Regulations at the Subcellular Level through Intracellular Polymerization, Assembly, and Transformation.JACS Au · 2024Review
- Rapid binding to protofilament edge sites facilitates tip tracking of EB1 at growing microtubule plus-ends.eLife · 2024Article
- Measuring and modeling forces generated by microtubules.Biophysical reviews · 2023Review
- Taxol acts differently on different tubulin isotypes.Communications biology · 2023Article
- Tubb3 expression levels are sensitive to neuronal activity changes and determine microtubule growth and kinesin-mediated transport.Cellular and molecular life sciences : CMLS · 2022Article
- Morphogenesis Dynamics in Leishmania Differentiation.Pathogens (Basel, Switzerland) · 2022Article
- Melanoma therapeutics: a literature review.Journal of biomedical research · 2022Article
- Regulation of microtubule dynamics, mechanics and function through the growing tip.Nature reviews. Molecular cell biology · 2021Review
- Transcriptome Profile Analysis of Triple-Negative Breast Cancer Cells in Response to a Novel Cytostatic Tetrahydroisoquinoline Compared to Paclitaxel.International journal of molecular sciences · 2021Article
- Atomistic Basis of Microtubule Dynamic Instability Assessed Via Multiscale Modeling.Annals of biomedical engineering · 2021Article
- Metabolomics Analyses Provide Insights Into Nutritional Value and Abiotic Stress Tolerance in HalophyteFrontiers in plant science · 2021Article
- Kinetically Stabilizing Mutations in Beta Tubulins Create Isotype-Specific Brain Malformations.Frontiers in cell and developmental biology · 2021Article
- Tau Avoids the GTP Cap at Growing Microtubule Plus-Ends.iScience · 2020Article
- Predicting Confined 1D Cell Migration from Parameters Calibrated to a 2D Motor-Clutch Model.Biophysical journal · 2020Article
- Numerical Parameter Space Compression and Its Application to Biophysical Models.Biophysical journal · 2020Article
- Taxanes convert regions of perturbed microtubule growth into rescue sites.Nature materials · 2020Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Microtubule-targeting agents (MTAs), widely used as biological probes and chemotherapeutic drugs, bind directly to tubulin subunits and "kinetically stabilize" microtubules, suppressing the characteristic self-assembly process of dynamic instability. However, the molecular-level mechanisms of kinetic stabilization are unclear, and the fundamental thermodynamic and kinetic requirements for dynamic instability and its elimination by MTAs have yet to be defined. Here we integrate a computational model for microtubule assembly with nanometer-scale fluorescence microscopy measurements to identify the kinetic and thermodynamic basis of kinetic stabilization by the MTAs paclitaxel, an assembly promoter, and vinblastine, a disassembly promoter. We identify two distinct modes of kinetic stabilization in live cells, one that truly suppresses on-off kinetics, characteristic of vinblastine, and the other a "pseudo" kinetic stabilization, characteristic of paclitaxel, that nearly eliminates the energy difference between the GTP- and GDP-tubulin thermodynamic states. By either mechanism, the main effect of both MTAs is to effectively stabilize the microtubule against disassembly in the absence of a robust GTP cap.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.