ArticleCell reports2017
Measurement of Rapid Protein Diffusion in the Cytoplasm by Photo-Converted Intensity Profile Expansion.
Article in Cell reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 49 citations in OpenAlex.
- Geometry effects on protein mobility in a synapse.Biophysical journal · 2025Article
- Advection versus diffusion in brain ventricular transport.Fluids and barriers of the CNS · 2025Article
- Integrative biophysical characterization of molecular interactions: A case study with the sfGFP-nanobody complex.Analytical biochemistry · 2025Article
- Impact of physiological ionic strength and crowding on kinesin-1 motility.Cell structure and function · 2025Article
- Article
- Natural convection in the cytoplasm: Theoretical predictions of buoyancy-driven flows inside a cell.PloS one · 2024Article
- Self-organized computation in the far-from-equilibrium cell.Biophysics reviews · 2022Review
- The BrightEyes-TTM as an open-source time-tagging module for democratising single-photon microscopy.Nature communications · 2022Article
- Single-molecule imaging reveals Tau trapping at nanometer-sized dynamic hot spots near the plasma membrane that persists after microtubule perturbation and cholesterol depletion.The EMBO journal · 2022Article
- Article
- Dynamic Mode Decomposition of Fluorescence Loss in Photobleaching Microscopy Data for Model-Free Analysis of Protein Transport and Aggregation in Living Cells.Sensors (Basel, Switzerland) · 2022Article
- Forces generated by lamellipodial actin filament elongation regulate the WAVE complex during cell migration.Nature cell biology · 2021Article
- Resveratrol and related stilbene derivatives induce stress granules with distinct clearance kinetics.Molecular biology of the cell · 2021Article
- Quantitative photoconversion analysis of internal molecular dynamics in stress granules and other membraneless organelles in live cells.STAR protocols · 2020Article
- Fasnall Induces Atypically Transient Stress Granules Independently of FASN Inhibition.iScience · 2020Article
- A comparative analysis of the mobility of 45 proteins in the synaptic bouton.The EMBO journal · 2020Article
- Unrestrained ESCRT-III drives micronuclear catastrophe and chromosome fragmentation.Nature cell biology · 2020Article
- Back to the Future: Genetically Encoded Fluorescent Proteins as Inert Tracers of the Intracellular Environment.International journal of molecular sciences · 2020Review
- Water is a biomarker of changes in the cellular environment in live animals.Scientific reports · 2020Article
- How Nanopore Translocation Experiments Can Measure RNA Unfolding.Biophysical journal · 2020Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The fluorescence microscopy methods presently used to characterize protein motion in cells infer protein motion from indirect observables, rather than measuring protein motion directly. Operationalizing these methods requires expertise that can constitute a barrier to their broad utilization. Here, we have developed PIPE (photo-converted intensity profile expansion) to directly measure the motion of tagged proteins and quantify it using an effective diffusion coefficient. PIPE works by pulsing photo-convertible fluorescent proteins, generating a peaked fluorescence signal at the pulsed region, and analyzing the spatial expansion of the signal. We demonstrate PIPE's success in measuring accurate diffusion coefficients in silico and in vitro and compare effective diffusion coefficients of native cellular proteins and free fluorophores in vivo. We apply PIPE to measure diffusion anomality in the cell and use it to distinguish free fluorophores from native cellular proteins. PIPE's direct measurement and ease of use make it appealing for cell biologists.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.