ArticleStem cells translational medicine2017
Human Lung Spheroids as In Vitro Niches of Lung Progenitor Cells with Distinctive Paracrine and Plasticity Properties.
Article in Stem cells translational medicine, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed.
- Next-Generation In Vitro Pulmonary Platforms for Respiratory Disease Modelling and Therapeutic Development: Current Advances and Future Prospects.Medicina (Kaunas, Lithuania) · 2026Review
- Lung organoids: a new frontier in neonatology and paediatric respiratory medicine.European respiratory review : an official journal of the European Respiratory Society · 2025Review
- Recent Advances in 3D Cultures.International journal of molecular sciences · 2024Article
- In vitro modelling of bacterial pneumonia: a comparative analysis of widely applied complex cell culture models.FEMS microbiology reviews · 2024Review
- The evolution ofEuropean respiratory review : an official journal of the European Respiratory Society · 2024Review
- Distinct bacterial population dynamics and disease dissemination after biofilm dispersal and disassembly.The ISME journal · 2023Article
- Organotypic Models of Lung Cancer.Current topics in microbiology and immunology · 2021Article
- Recapitulating TumorigenesisFrontiers in bioengineering and biotechnology · 2021Review
- Inhibition of miR-155 Attenuates Detrimental Vascular Effects of Tobacco Cigarette Smoking.Journal of the American Heart Association · 2020Article
- A pre-investigational new drug study of lung spheroid cell therapy for treating pulmonary fibrosis.Stem cells translational medicine · 2020Article
- Cartilage and bone tissue engineering using adipose stromal/stem cells spheroids as building blocks.World journal of stem cells · 2020Review
- Where We Stand: Lung Organotypic Living Systems That Emulate Human-Relevant Host-Environment/Pathogen Interactions.Frontiers in bioengineering and biotechnology · 2020Review
- Improving the viability of tissue-resident stem cells using an organ-preservation solution.FEBS open bio · 2019Article
- Lung Injury Repair by Transplantation of Adult Lung Cells Following Preconditioning of Recipient Mice.Stem cells translational medicine · 2018Article
- EMT/MET at the Crossroad of Stemness, Regeneration and Oncogenesis: The Ying-Yang Equilibrium Recapitulated in Cell Spheroids.Cancers · 2017Review
- Derivation of therapeutic lung spheroid cells from minimally invasive transbronchial pulmonary biopsies.Respiratory research · 2017Article
- Stem Cell Spheroids and Ex Vivo Niche Modeling: Rationalization and Scaling-Up.Journal of cardiovascular translational research · 2017Review
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Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Basic and translational research on lung biology has discovered multiple progenitor cell types, specialized or facultative, responsible for turnover, renewal, and repair. Isolation of populations of resident lung progenitor cells (LPCs) has been described by multiple protocols, and some have been successfully applied to healthy human lung tissue. We aimed at understanding how different cell culture conditions may affect, in vitro, the phenotype of LPCs to create an ideal niche-like microenvironment. The influence of different substrates (i.e., fibronectin, gelatin, laminin) and the impact of a three-dimensional/two-dimensional (3D/2D) culture switch on the biology of LPCs isolated as lung spheroids (LSs) from normal adult human lung biopsy specimens were investigated. We applied a spheroid culture system as the selective/inductive step for progenitor cell culture, as described in many biological systems. The data showed a niche-like proepithelial microenvironment inside the LS, highly sensitive to the 3D culture system and significantly affecting the phenotype of adult LPCs more than culture substrate. LSs favor epithelial phenotypes and LPC maintenance and contain cells more responsive to specific commitment stimuli than 2D monolayer cultures, while secreting a distinctive set of paracrine factors. We have shown for the first time, to our knowledge, how culture as 3D LSs can affect LPC epithelial phenotype and produce strong paracrine signals with a distinctive secretomic profile compared with 2D monolayer conditions. These findings suggest novel approaches to maintain ex vivo LPCs for basic and translational studies. Stem Cells Translational Medicine 2017;6:767-777.
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