Evidence map›Paper›PMID 28297567›Full record

ArticleStem cells translational medicine2017

Developing a Novel Two-Dimensional Culture System to Enrich Human Prostate Luminal Progenitors that Can Function as a Cell of Origin for Prostate Cancer.

Dingxiao Zhang, Kevin Lin, Yue Lu, Kiera Rycaj, Yi Zhong, Hsueh-Ping Chao, Tammy Calhoun-Davis, Jianjun Shen, Dean G Tang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cells translational medicine, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Review
  6. Article
  7. Review
  8. Article
  9. Prostate Stem Cells and Cancer Stem Cells.Cold Spring Harbor perspectives in medicine · 2019
    Review
  10. Article
  11. Tumor Dormancy and Slow-Cycling Cancer Cells.Advances in experimental medicine and biology · 2019
    Review
  12. Review
  13. Review
  14. Article
  15. Article
  16. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Dingxiao ZhangDepartment of Epigenetics and Molecular Carcinogenesis, University of Texas, MD Anderson Cancer Center, Houston, Texas, USA.
Kevin LinDepartment of Epigenetics and Molecular Carcinogenesis, University of Texas, MD Anderson Cancer Center, Houston, Texas, USA.
Yue LuDepartment of Epigenetics and Molecular Carcinogenesis, University of Texas, MD Anderson Cancer Center, Houston, Texas, USA.
Kiera RycajDepartment of Epigenetics and Molecular Carcinogenesis, University of Texas, MD Anderson Cancer Center, Houston, Texas, USA.
Yi ZhongDepartment of Epigenetics and Molecular Carcinogenesis, University of Texas, MD Anderson Cancer Center, Houston, Texas, USA.
Hsueh-Ping ChaoDepartment of Epigenetics and Molecular Carcinogenesis, University of Texas, MD Anderson Cancer Center, Houston, Texas, USA.
Tammy Calhoun-DavisDepartment of Epigenetics and Molecular Carcinogenesis, University of Texas, MD Anderson Cancer Center, Houston, Texas, USA.
Jianjun ShenDepartment of Epigenetics and Molecular Carcinogenesis, University of Texas, MD Anderson Cancer Center, Houston, Texas, USA.
Dean G TangDepartment of Epigenetics and Molecular Carcinogenesis, University of Texas, MD Anderson Cancer Center, Houston, Texas, USA.

Funding

Decoding NanogP8 in TumorigenesisR01CA155693 · NCI · UNIVERSITY OF TX MD ANDERSON CAN CTR · PI TANG, DEAN G. · 2012 to 2016
$1.6M
NCI NIH HHS R01 CA155693
6 · The paper itself

Abstract

Elucidating the cell of origin of cancer has great significance in stratifying patients into appropriate treatment groups and for developing novel targeted therapies. Early studies demonstrate that only stem-like basal cells in the normal human prostate (NHP) can function as the cell of origin for prostate cancer (PCa). Here, we show that the organoids derived from bulk NHP luminal cells can also be tumorigenically transformed. We further show that the WIT medium, which is used to culture human mammary epithelial progenitor cells, when combined with the ROCK inhibitor, can readily propagate a population of progenitor-like cells from the primary NHP luminal cell isolates. Such functionally defined luminal progenitors can be transformed by distinct sets of genetic perturbations (i.e., AR+AKT/ERG or c-MYC+PTEN knockout) to form tumor glands. Genome-wide RNA-Seq analysis of freshly purified unperturbed human benign prostatic basal and luminal cells and culture-expanded lineage-specific stem/progenitor populations reveals that the luminal progenitors possess a distinct gene expression profile that is greatly enriched in advanced, castration-resistant, and metastatic PCa, and it associates with poor patient survival. The ability of the simple two-dimensional culture system reported herein to greatly enrich NHP progenitor-like cells should facilitate biological and biochemical studies as well as high-throughput screening in these cells and in progenitor-like PCa cells. Stem Cells Translational Medicine 2017;6:748-760.

Indexed as

CastrationCell Culture TechniquesCell LineageCells, CulturedCell Transformation, NeoplasticCulture MediaEpithelial CellsGene Expression Regulation, NeoplasticHumansMaleNeoplasm InvasivenessNeoplasm MetastasisOrganoidsProstateProstatic NeoplasmsRegenerationCulture MediaCancer cell of originProstate cancerProstate epithelial progenitor cellsStem cells

Identifiers

PMID28297567
PMCPMC5442765

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.