ArticlePloS one2017
An oral multispecies biofilm model for high content screening applications.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
41 citing papers in PubMed, 1 synthesis or guideline pooled it, 66 citations in OpenAlex.
- Better models, better treatment? a systematic review of current three dimensional (3D)Frontiers in bioengineering and biotechnology · 2025Pooled it
- Quantitative Assessment of Primary Colonizer Adhesion on Different Resin-Based Restorative Materials Using SYBR Green qPCR.Dentistry journal · 2026Article
- Establishment of a three-dimensional in vitro peri-implant bone-mucosa composite model.BMC oral health · 2026Article
- The impact of DNA extraction methods on species quantification and apparent community composition of in vitro oral biofilms.FEMS microbiology letters · 2026Article
- Synergistic potential of antibiotics against anFrontiers in bioengineering and biotechnology · 2026Article
- Laser-assisted microbial culturomics.Nature communications · 2025Article
- Unraveling novel insights into dual-species cariogenic biofilm formation on aged teeth: a comparative analysis on natural vs artificial bioengineered dentin models.Applied and environmental microbiology · 2025Article
- The Effect of Chlorhexidine Mouthwashes on the Microbiota Associated with Peri-Implantitis Lesions: A Pilot Study.Antibiotics (Basel, Switzerland) · 2025Article
- Impact of antibacterial therapeutic agents on biofilm-tissue interactions in a 3D implant-tissue-oral-bacterial-biofilm model.Scientific reports · 2025Article
- Dual Antibacterial and Soft-Tissue-Integrative Effect of Combined Strontium Acetate and Silver Nitrate on Peri-Implant Environment: Insights from Multispecies Biofilms and a 3D Coculture Model.ACS applied materials & interfaces · 2025Article
- Influence of species composition and cultivation condition on peri-implant biofilm dysbiosisFrontiers in oral health · 2025Article
- Synergistic effects of biofilm-producing PGPR strains on wheat plant colonization, growth and soil resilience under drought stress.Saudi journal of biological sciences · 2023Article
- Effect of titanium implants along with silver ions and tetracycline on type I interferon-beta expression during implant-related infections in co-culture and mouse model.Frontiers in bioengineering and biotechnology · 2023Article
- Comparison ofBioMed research international · 2023Article
- Effects of Preservation and Propagation Methodology on Microcosms Derived from the Oral Microbiome.Microorganisms · 2022Article
- Silver Nanoparticles Produced by Laser Ablation and Re-Irradiation Are Effective Preventing Peri-Implantitis Multispecies Biofilm Formation.International journal of molecular sciences · 2022Article
- Succession of oral bacterial colonizers on dental implant materials: An in vitro biofilm model.Dental materials : official publication of the Academy of Dental Materials · 2022Article
- Article
- Low-Temperature Plasma Short Exposure to Decontaminate Peri-Implantitis-Related Multispecies Biofilms on Titanium SurfacesBioMed research international · 2022Article
- A novel chlorhexidine-hexametaphosphate coating for titanium with antibiofilm efficacy and stem cell cytocompatibility.Journal of materials science. Materials in medicine · 2021Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Peri-implantitis caused by multispecies biofilms is a major complication in dental implant treatment. The bacterial infection surrounding dental implants can lead to bone loss and, in turn, to implant failure. A promising strategy to prevent these common complications is the development of implant surfaces that inhibit biofilm development. A reproducible and easy-to-use biofilm model as a test system for large scale screening of new implant surfaces with putative antibacterial potency is therefore of major importance. In the present study, we developed a highly reproducible in vitro four-species biofilm model consisting of the highly relevant oral bacterial species Streptococcus oralis, Actinomyces naeslundii, Veillonella dispar and Porphyromonas gingivalis. The application of live/dead staining, quantitative real time PCR (qRT-PCR), scanning electron microscopy (SEM) and urea-NaCl fluorescence in situ hybridization (urea-NaCl-FISH) revealed that the four-species biofilm community is robust in terms of biovolume, live/dead distribution and individual species distribution over time. The biofilm community is dominated by S. oralis, followed by V. dispar, A. naeslundii and P. gingivalis. The percentage distribution in this model closely reflects the situation in early native plaques and is therefore well suited as an in vitro model test system. Furthermore, despite its nearly native composition, the multispecies model does not depend on nutrient additives, such as native human saliva or serum, and is an inexpensive, easy to handle and highly reproducible alternative to the available model systems. The 96-well plate format enables high content screening for optimized implant surfaces impeding biofilm formation or the testing of multiple antimicrobial treatment strategies to fight multispecies biofilm infections, both exemplary proven in the manuscript.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.