ArticlePloS one2017
Blue-light induced accumulation of reactive oxygen species is a consequence of the Drosophila cryptochrome photocycle.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.
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Who cites it
27 citing papers in PubMed, 1 synthesis or guideline pooled it, 57 citations in OpenAlex.
- Genetic analysis of cryptochrome in insect magnetosensitivity.Frontiers in physiology · 2022Pooled it
- Spectroscopic Characterization of Radical Pair Photochemistry in Nonmigratory Avian Cryptochromes: Magnetic Field Effects inJournal of the American Chemical Society · 2025Article
- Control of odor sensation by light and cryptochrome in theiScience · 2025Article
- Hidden route of protein damage through oxygen-confined photooxidation.Nature communications · 2024Article
- Magnetosensitivity of tightly bound radical pairs in cryptochrome is enabled by the quantum Zeno effect.Nature communications · 2024Article
- Heat-inactivated Streptococcus pneumoniae augments circadian clock gene expression in zebrafish cells.Scientific reports · 2024Article
- Exposure of Bladder Cancer Cells to Blue Light (λ = 453 nm) in the Presence of Riboflavin Synergistically Enhances the Cytotoxic Efficiency of Gemcitabine.International journal of molecular sciences · 2024Article
- Quantitative measurements of reactive oxygen species partitioning in electron transfer flavoenzyme magnetic field sensing.Frontiers in physiology · 2024Article
- Differential regulation of sleep by blue, green, and red light inFrontiers in behavioral neuroscience · 2024Article
- A structural decryption of cryptochromes.Frontiers in chemistry · 2024Review
- Magnetic Field Intervention Enhances Cellular Migration Rates in Biological Scaffolds.Bioengineering (Basel, Switzerland) · 2023Article
- Could violet-blue lights increase the bacteria resistance against ultraviolet radiation mediated by photolyases?Lasers in medical science · 2023Review
- Zeaxanthin is required for eyespot formation and phototaxis in Euglena gracilis.Plant physiology · 2023Article
- Cryptochromes in mammals: a magnetoreception misconception?Frontiers in physiology · 2023Review
- Human magnetic sense is mediated by a light and magnetic field resonance-dependent mechanism.Scientific reports · 2022Article
- Blue light responses in Cancer borealis stomatogastric ganglion neurons.Current biology : CB · 2022Article
- Article
- Radical pairs can explain magnetic field and lithium effects on the circadian clock.Scientific reports · 2022Article
- A little goes a long way: Neurobiological effects of low intensity rTMS and implications for mechanisms of rTMS.Current research in neurobiology · 2022Review
- Selective induction of rapid cytotoxic effect in glioblastoma cells by oscillating magnetic fields.Journal of cancer research and clinical oncology · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
15 authors at 9 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cryptochromes are evolutionarily conserved blue-light absorbing flavoproteins which participate in many important cellular processes including in entrainment of the circadian clock in plants, Drosophila and humans. Drosophila melanogaster cryptochrome (DmCry) absorbs light through a flavin (FAD) cofactor that undergoes photoreduction to the anionic radical (FAD•-) redox state both in vitro and in vivo. However, recent efforts to link this photoconversion to the initiation of a biological response have remained controversial. Here, we show by kinetic modeling of the DmCry photocycle that the fluence dependence, quantum yield, and half-life of flavin redox state interconversion are consistent with the anionic radical (FAD•-) as the signaling state in vivo. We show by fluorescence detection techniques that illumination of purified DmCry results in enzymatic conversion of molecular oxygen (O2) to reactive oxygen species (ROS). We extend these observations in living cells to demonstrate transient formation of superoxide (O2•-), and accumulation of hydrogen peroxide (H2O2) in the nucleus of insect cell cultures upon DmCry illumination. These results define the kinetic parameters of the Drosophila cryptochrome photocycle and support light-driven electron transfer to the flavin in DmCry signaling. They furthermore raise the intriguing possibility that light-dependent formation of ROS as a byproduct of the cryptochrome photocycle may contribute to its signaling role.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.