ArticleNeurogastroenterology and motility2017
Assessing the colonic microbiome, hydrogenogenic and hydrogenotrophic genes, transit and breath methane in constipation.
Article in Neurogastroenterology and motility, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 1 synthesis or guideline pooled it, 29 citations in OpenAlex.
- Research on functional constipation with anxiety or depression: a bibliometric analysis.Frontiers in psychiatry · 2025Pooled it
- Altered Gut Microbic Flora and Haemorrhoids: Could They Have a Possible Relationship?Journal of clinical medicine · 2023Article
- Interpreting tree ensemble machine learning models with endoR.PLoS computational biology · 2022Article
- Association between Depression, Anxiety Symptoms and Gut Microbiota in Chinese Elderly with Functional Constipation.Nutrients · 2022Article
- Analysis of Key Genes for Slow Transit Constipation Based on RNA Sequencing.International journal of general medicine · 2022Article
- Interaction between the Gut Microbiota and Intestinal Motility.Evidence-based complementary and alternative medicine : eCAM · 2022Review
- Understanding the physiology of human defaecation and disorders of continence and evacuation.Nature reviews. Gastroenterology & hepatology · 2021Review
- Review article: diagnosis, management and patient perspectives of the spectrum of constipation disorders.Alimentary pharmacology & therapeutics · 2021Review
- [Influencing Factors on Lactulose Breath Test Results].The Korean journal of gastroenterology = Taehan Sohwagi Hakhoe chi · 2020Article
- Mucosal-Associated Microbiota Other Than Luminal Microbiota Has a Close Relationship With Diarrhea-Predominant Irritable Bowel Syndrome.Frontiers in cellular and infection microbiology · 2020Article
- More movement with evaluating colonic transit in humans.Neurogastroenterology and motility · 2019Review
- Hydrogen cross-feeders of the human gastrointestinal tract.Gut microbes · 2019Review
- The Gut Microbiome in Adult and Pediatric Functional Gastrointestinal Disorders.Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association · 2019Review
- Distinct microbes, metabolites, and ecologies define the microbiome in deficient and proficient mismatch repair colorectal cancers.Genome medicine · 2018Article
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 2 countries.
Funding
Abstract
backgroundDifferences in the gut microbiota and breath methane production have been observed in chronic constipation, but the relationship between colonic microbiota, transit, and breath tests remains unclear.
methodsIn 25 healthy and 25 constipated females we evaluated the sigmoid colonic mucosal and fecal microbiota using 16S rRNA gene sequencing, abundance of hydrogenogenic FeFe (FeFe-hydA) and hydrogenotrophic (methyl coenzyme M reductase A [mrcA] and dissimilatory sulfite reductase A [dsrA]) genes with real-time qPCR assays, breath hydrogen and methane levels after oral lactulose, and colonic transit with scintigraphy. KEY
resultsBreath hydrogen and methane were not correlated with constipation, slow colon transit, or with abundance of corresponding genes. After adjusting for colonic transit, the abundance of FeFehydA, dsrA, and mcrA were greater (P<.005) in colonic mucosa, but not stool, of constipated patients. The abundance of the selected functional gene targets also correlated with that of selected taxa. The colonic mucosal abundance of FeFe-hydA, but not mcrA, correlated positively (P<.05) with breath methane production, slow colonic transit, and overall microbiome composition. In the colonic mucosa and feces, the abundance of hydrogenogenic and hydrogenotrophic genes were positively correlated (P<.05). Breath methane production was not associated with constipation or colonic transit. CONCLUSIONS & INFERENCES: Corroborating our earlier findings with 16S rRNA genes, colonic mucosal but not fecal hydrogenogenic and hydrogenotrophic genes were more abundant in constipated vs. healthy subjects independent of colonic transit. Breath gases do not directly reflect the abundance of target genes contributing to their production.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.