ArticleScientific reports2017
In vivo imaging of the Muc5b gel-forming mucin.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 1 synthesis or guideline pooled it, 30 citations in OpenAlex.
- Vaginal probiotics as therapeutic adjuncts for improving embryo transfer success rates: a systematic review and meta-analysis.BMC pregnancy and childbirth · 2025Pooled it
- Ovarian sex steroid and epithelial control of immune responses in the uterus and oviduct: human and animal models†.Biology of reproduction · 2024Article
- Immunohistochemical detection of MUC5AC and MUC5B mucins in ferrets.BMC research notes · 2023Article
- Dynamic states of cervical epithelia during pregnancy and epithelial barrier disruption.iScience · 2023Article
- A porous cervical mucus plug leads to preterm birth induced by experimental vaginal infection in mice.iScience · 2022Article
- Transcriptome and proteome dynamics of cervical remodeling in the mouse during pregnancy†.Biology of reproduction · 2021Article
- Pharyngeal Deposits Comprising Salivary Mucin in Tube-fed Elderly Patients: MUC2 and MUC7 Immunoreactivity.Dysphagia · 2021Article
- The Cervicovaginal Mucus Barrier.International journal of molecular sciences · 2020Review
- Role ofFrontiers in cellular and infection microbiology · 2020Review
- ILC2s mediate systemic innate protection by priming mucus production at distal mucosal sites.The Journal of experimental medicine · 2019Article
- Muc5b-deficient mice develop early histological lung abnormalities.Biology open · 2019Article
- Muc5b is mainly expressed and sialylated in the nasal olfactory epithelium whereas Muc5ac is exclusively expressed and fucosylated in the nasal respiratory epithelium.Histochemistry and cell biology · 2019Article
- Mucins and Pathogenic Mucin-Like Molecules Are Immunomodulators During Infection and Targets for Diagnostics and Vaccines.Frontiers in chemistry · 2019Review
- Preclinical mouse model to monitor live Muc5b-producing conjunctival goblet cell density under pharmacological treatments.PloS one · 2017Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gel-forming mucins are macromolecules produced by goblet cells and responsible for the mucus gel formation. Changes in goblet cell density and in gel-forming mucin production have emerged as sensitive indicators for mucosal diseases. A Muc5b-GFP tagged reporter mouse was used to assess Muc5b production in mouse tissues by immunofluorescence microscopy and fluorescent activity using stereromicroscopy and probe-based confocal laser endomicroscopy. Muc5b production was followed longitudinally by recording the fluorescent activity in vagina and in embryonic lung explants under stimulation by interleukin 13. We show that the GFP is easily visualized in the mouse adult ear, nose, trachea, gallbladder, and cervix. Live Muc5b is also easily monitored in the nasal cavity, trachea and vagina where its production varies during the estrus cycle with a peak at the proestrus phase and in pregnant mice. Explant culture of reporter mouse embryonic whole lung shows that interleukin 13 stimulates Muc5b production. The transgenic Muc5b-GFP mouse is unique and suitable to study the mechanisms that regulate Muc5b production/secretion and mucous cell differentiation by live imaging and can be applied to test drug efficacy in mucosal disease models.
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