ArticlePlanta2017
In vitro polyploidy induction: changes in morphology, podophyllotoxin biosynthesis, and expression of the related genes in Linum album (Linaceae).
Article in Planta, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 88 citations in OpenAlex.
- In vitro induction of tetraploids and their morphological and phenolic acid content in Cnidium officinale.BMC plant biology · 2026Article
- Induction and phenotype analysis of autotetraploids of Morus Mongolica.BMC plant biology · 2026Article
- Induction of autotetraploid and morphological, anatomical, and cytological studies of Trichosanthes cucumerina Linn.BMC plant biology · 2025Article
- Genetic Diversity Analysis and Polyploid Induction Identification ofPlants (Basel, Switzerland) · 2024Article
- Genomic characterization and identification of candidate genes for putative podophyllotoxin biosynthesis pathway in Penicillium herquei HGN12.1C.Microbial biotechnology · 2024Article
- The effect of induced polyploidy on phytochemistry, cellular organelles and the expression of genes involved in thymol and carvacrol biosynthetic pathway in thyme (Frontiers in plant science · 2023Article
- Mixed-Ploidy and Dysploidy inPlants (Basel, Switzerland) · 2022Article
- Transcriptome analyses reveal the expression profile of genes related to lignan biosynthesis inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2022Article
- Physiological, biochemical, and molecular responses ofPhysiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
- Effect of Polyploidy Induction on Natural Metabolite Production in Medicinal Plants.Biomolecules · 2021Review
- Induced autopolyploidy-a promising approach for enhanced biosynthesis of plant secondary metabolites: an insight.Journal, genetic engineering & biotechnology · 2021Review
- Article
- Article
- Synthetic Polyploidy in Grafted Crops.Frontiers in plant science · 2020Review
- Review
- Gene body demethylation increases expression and is associated with self-pruning during grape genome duplication.Horticulture research · 2020Article
- Review
- Improvement of growth and bacoside production inPeerJ · 2019Article
- Assessment of Iranian endemic Artemisia khorassanica: karyological, genome size, and gene expressions involved in artemisinin production.Turkish journal of biology = Turk biyoloji dergisi · 2018Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MAIN
conclusionInduction of tetraploidy was performed and podophyllotoxin production increased by upregulating the expression level and enzyme activity of genes related to its biosynthesis in tetraploid compared to diploid Linum album. Linum album is a valuable medicinal plant that produces antiviral and anticancer compounds including podophyllotoxin (PTOX). To achieve homogeneous materials, in vitro diploid clones were established, and their nodal segments were exposed to different concentrations and durations of colchicine. This resulted in successful tetraploidy induction, confirmed by flow cytometry, and is being reported for the first time. The highest efficiency of tetraploid induction (22%) was achieved after 72 h exposure to 2.5-mM colchicine treatment. The stable tetraploids were produced after being subcultured three times, and their ploidy stability was confirmed after each subculture. The effects of autopolyploidy were measured on the morphological and phytochemical characteristics, as well as enzyme activity and the expression levels of some key genes involved in the PTOX biosynthetic pathway, including phenylalanine ammonia-lyase (PAL), cinnamoyl-Coa reductase (CCR), cinnamyl-alcohol dehydrogenase (CAD), and pinoresinol-lariciresinol reductase (PLR). The tetraploid plants had larger leaves and stomata (length and width) and lower density stomata. Increasing the ploidy level from diploid to tetraploid resulted in 1.39- and 1.23-fold enhancement of PTOX production, respectively, in the leaves and stem. The increase in PTOX content was associated with upregulated activities of some enzymes studied related to its biosynthetic pathway and the expression of the corresponding genes. The expression of the PAL gene and PLR enzymatic activity had the most positive correlation with the ploidy level in both leaf and stem tissues. Our results verified that autotetraploid induction is a useful breeding method, remarkably increasing the PTOX content in the leaves and stem of L. album.
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