Evidence map›Paper›PMID 28293464›Full record

ArticleCell medicine2017

Measurement of DNA Length Changes Upon CpG Hypermethylation by Microfluidic Molecular Stretching.

Daisuke Onoshima, Naoko Kawakita, Daiki Takeshita, Hirohiko Niioka, Hiroshi Yukawa, Jun Miyake, Yoshinobu Baba

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell medicine, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Daisuke OnoshimaInstitute of Innovation for Future Society, Nagoya University, Chikusa-ku, Nagoya, Japan; †ImPACT Research Center for Advanced Nanobiodevices, Nagoya University, Chikusa-ku, Nagoya, Japan.
Naoko Kawakita†ImPACT Research Center for Advanced Nanobiodevices, Nagoya University, Chikusa-ku, Nagoya, Japan; ‡Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Chikusa-ku, Nagoya, Japan.
Daiki Takeshita†ImPACT Research Center for Advanced Nanobiodevices, Nagoya University, Chikusa-ku, Nagoya, Japan; ‡Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Chikusa-ku, Nagoya, Japan.
Hirohiko Niioka§ Graduate School of Engineering Science, Osaka University , Toyonaka, Osaka , Japan.
Hiroshi Yukawa†ImPACT Research Center for Advanced Nanobiodevices, Nagoya University, Chikusa-ku, Nagoya, Japan; ‡Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Chikusa-ku, Nagoya, Japan.
Jun Miyake§ Graduate School of Engineering Science, Osaka University , Toyonaka, Osaka , Japan.
Yoshinobu BabaInstitute of Innovation for Future Society, Nagoya University, Chikusa-ku, Nagoya, Japan; †ImPACT Research Center for Advanced Nanobiodevices, Nagoya University, Chikusa-ku, Nagoya, Japan; ‡Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Chikusa-ku, Nagoya, Japan; ¶Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Takamatsu, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Abnormal DNA methylation in CpG-rich promoters is recognized as a distinct molecular feature of precursor lesions to cancer. Such unintended methylation can occur during in vitro differentiation of stem cells. It takes place in a subset of genes during the differentiation or expansion of stem cell derivatives under general culture conditions, which may need to be monitored in future cell transplantation studies. Here we demonstrate a microfluidic device for investigating morphological length changes in DNA methylation. Arrayed polymer chains of single DNA molecules were fluorescently observed by parallel trapping and stretching in the microfluidic channel. This observational study revealed that the shortened DNA length is due to the increased rigidity of the methylated DNA molecule. The trapping rate of the device for DNA molecules was substantially unaffected by changes in the CpG methylation.

Indexed as

Cytosine-guanine dinucleotides (CpG)DNA methylationMicrofluidic deviceSingle-molecule detection

Identifiers

PMID28293464
PMCPMC5225679

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.