ArticleEpigenetics & chromatin2017
Silencing markers are retained on pericentric heterochromatin during murine primordial germ cell development.
Article in Epigenetics & chromatin, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 28 citations in OpenAlex.
- Global reorganization of genome architecture at the transition to gametogenesis.Nature structural & molecular biology · 2026Article
- Chromatin accessibility landscapes define stromal cell identities across tissues.Communications biology · 2026Article
- IDH2 and GLUD1 depletion arrests embryonic development through an H4K20me3 epigenetic barrier in porcine parthenogenetic embryos.Zoological research · 2024Article
- Reinforcement of repressive marks in the chicken primordial germ cell epigenetic signature: divergence from basal state resetting in mammals.Epigenetics & chromatin · 2024Article
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- ATF7-dependent epigenetic changes induced by high temperature during early porcine embryonic development.Cell proliferation · 2023Article
- Hippo signaling and histone methylation control cardiomyocyte cell cycle re-entry through distinct transcriptional pathways.PloS one · 2023Article
- Bend family proteins mark chromatin boundaries and synergistically promote early germ cell differentiation.Protein & cell · 2022Article
- Interplay between chromatin marks in development and disease.Nature reviews. Genetics · 2022Review
- ADAD2 regulates heterochromatin in meiotic and post-meiotic male germ cells via translation of MDC1.Journal of cell science · 2022Article
- Global Gene Expression Regulation Mediated by TGFβ Through H3K9me3 Mark.Cancer informatics · 2022Article
- The repair geneOncology reviews · 2021Article
- Alteration of active and repressive histone marks during adipogenic differentiation of porcine mesenchymal stem cells.Scientific reports · 2021Article
- Bioinformatics analysis of the network of histone H3 lysine 9 trimethylation in acute myeloid leukaemia.Oncology reports · 2020Article
- Parental Effects on Epigenetic Programming in Gametes and Embryos of Dairy Cows.Frontiers in genetics · 2020Review
- Expression dynamics of repetitive DNA in early human embryonic development.BMC genomics · 2019Article
- The Emerging Role of H3K9me3 as a Potential Therapeutic Target in Acute Myeloid Leukemia.Frontiers in oncology · 2019Review
- Epigenetic and non-epigenetic mode of SIRT1 action during oocyte meiosis progression.Journal of animal science and biotechnology · 2019Article
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Authors and funding
9 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIn the nuclei of most mammalian cells, pericentric heterochromatin is characterized by DNA methylation, histone modifications such as H3K9me3 and H4K20me3, and specific binding proteins like heterochromatin-binding protein 1 isoforms (HP1 isoforms). Maintenance of this specialized chromatin structure is of great importance for genome integrity and for the controlled repression of the repetitive elements within the pericentric DNA sequence. Here we have studied histone modifications at pericentric heterochromatin during primordial germ cell (PGC) development using different fixation conditions and fluorescent immunohistochemical and immunocytochemical protocols.
resultsWe observed that pericentric heterochromatin marks, such as H3K9me3, H4K20me3, and HP1 isoforms, were retained on pericentric heterochromatin throughout PGC development. However, the observed immunostaining patterns varied, depending on the fixation method, explaining previous findings of a general loss of pericentric heterochromatic features in PGCs. Also, in contrast to the general clustering of multiple pericentric regions and associated centromeres in DAPI-dense regions in somatic cells, the pericentric regions of PGCs were more frequently organized as individual entities. We also observed a transient enrichment of the chromatin remodeler ATRX in pericentric regions in embryonic day 11.5 (E11.5) PGCs. At this stage, a similar and low level of major satellite repeat RNA transcription was detected in both PGCs and somatic cells.
conclusionsThese results indicate that in pericentric heterochromatin of mouse PGCs, only minor reductions in levels of some chromatin-associated proteins occur, in association with a transient increase in ATRX, between E11.5 and E13.5. These pericentric heterochromatin regions more frequently contain only a single centromere in PGCs compared to the surrounding soma, indicating a difference in overall organization, but there is no de-repression of major satellite transcription.
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