ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
A plate method for rapid screening of Ketogulonicigenium vulgare mutants for enhanced 2-keto-l-gulonic acid production.
Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Exogenous 2-Keto-L-Gulonic Acid Supplementation Promotes L-Ascorbic Acid Biosynthesis inInternational journal of molecular sciences · 2026Article
- Extremophiles in Space Exploration.Indian journal of microbiology · 2024Review
- Identification of Pathways for Production of D-Glucaric Acid by Pseudogluconobacter saccharoketogenes.Applied biochemistry and biotechnology · 2024Article
- Exogenous 2-keto-L-gulonic Acid Supplementation as a Novel Approach to Enhancing L-ascorbic Acid Biosynthesis in Zebrafish (Animals : an open access journal from MDPI · 2023Article
- 2-Keto-L-Gulonic Acid Improved the Salt Stress Resistance of Non-heading Chinese Cabbage by Increasing L-Ascorbic Acid Accumulation.Frontiers in plant science · 2021Article
- High Throughput Screening Platform for a FAD-Dependent L-Sorbose Dehydrogenase.Frontiers in bioengineering and biotechnology · 2020Article
- High-Throughput Screening of a 2-Keto-L-Gulonic Acid-ProducingFrontiers in bioengineering and biotechnology · 2019Article
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6 authors.
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Abstract
A new plate method was developed for rapid screening of Ketogulonicigenium vulgare mutants overproducing 2-keto-l-gulonic acid (2-KLG). The screening methodology took the advantage of the acidity caused by 2-KLG, which changes the color of bromothymol blue (pH indicator) from blue to yellow. Using the proposed method, a mutant, K. vulgare 65, was selected from 20,000 colonies produced by a strain subjected to spaceflight mutagenesis. When co-cultured with Bacillus megaterium 2980 in 20-L fermenters, K. vulgare 65 showed a high conversion rate (94.45%) of l-sorbose to 2-KLG. In contrast to the traditional screening method, this one significantly improved the frequency of obtaining positive mutants. The proposed plate screening method is cost-effective and easy to run and is thus useful for the isolation and screening of K. vulgare mutants overproducing 2-KLG.
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