Evidence map›Paper›PMID 28291900›Full record

ArticleJournal of applied biomaterials & functional materials2017

Study of mesenchymal stem cells cultured on a poly(lactic-co-glycolic acid) scaffold containing simvastatin for bone healing.

Dario Mendes Junior, Juliana A Domingues, Moema A Hausen, Silvia M M Cattani, Aguedo Aragones, Alexandre L R Oliveira, Rodrigo F Inácio, Maria L P Barbo, Eliana A R Duek

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of applied biomaterials & functional materials, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
2.4field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 24 citations in OpenAlex.

  1. Review
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  3. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Dario Mendes JuniorDepartment of Physiological Sciences, Biomaterials Laboratory, Pontifical Catholic University, Sorocaba, São Paulo - Brazil.
Juliana A DominguesDepartment of Physiological Sciences, Biomaterials Laboratory, Pontifical Catholic University, Sorocaba, São Paulo - Brazil.
Moema A HausenPost Graduation Program of Biotechnology and Environmental Monitoring, Federal University of Sao Carlos, Sorocaba, São Paulo - Brazil.
Silvia M M CattaniDepartment of Physiological Sciences, Biomaterials Laboratory, Pontifical Catholic University, Sorocaba, São Paulo - Brazil.
Aguedo AragonesDepartment of Materials Engineering, Faculty of Mechanical Engineering, University of Campinas, São Paulo - Brazil.
Alexandre L R OliveiraDepartment of Cell Biology and Structural Biology, Biology Institute, University of Campinas, São Paulo - Brazil.
Rodrigo F InácioDepartment of Cell Biology and Structural Biology, Biology Institute, University of Campinas, São Paulo - Brazil.
Maria L P BarboDepartment of Physiological Sciences, Biomaterials Laboratory, Pontifical Catholic University, Sorocaba, São Paulo - Brazil.
Eliana A R DuekDepartment of Physiological Sciences, Biomaterials Laboratory, Pontifical Catholic University, Sorocaba, São Paulo - Brazil.
Pontifícia Universidade Católica de São Paulo · BRUniversidade Estadual de Campinas (UNICAMP) · BRUniversidade Federal de São Carlos · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundTissue engineering is a promising alternative for the development of bone substitutes; for this purpose, three things are necessary: stem cells, a scaffold to allow tissue growth and factors that induce tissue regeneration.

methodsTo congregate such efforts, we used the bioresorbable and biocompatible polymer poly(lactic-co-glycolic acid) (PLGA) as scaffold. For the osteoinductive factor, we used simvastatin (SIM), a drug with a pleiotropic effect on bone growth. Mesenchymal stem cells (MSCs) were cultured in PLGA containing SIM, and the bone substitute of PLGA/SIM/MSC was grafted into critical defects of rat calvaria.

resultsThe in vitro results showed that SIM directly interfered with the proliferation of MSC promoting cell death, while in the pure PLGA scaffold the MSC grew continuously. Scaffolds were implanted in the calvaria of rats and separated into groups: control (empty defect), PLGA pure, PLGA/SIM, PLGA/MSC and PLGA/SIM/MSC. The increase in bone growth was higher in the PLGA/SIM group.

conclusionsWe observed no improvement in the growth of bone tissue after implantation of the PLGA/SIM/MSC scaffold. As compared with in vitro results, our main hypothesis is that the microarchitecture of PLGA associated with low SIM release would have created an in vivo microenvironment of concentrated SIM that might have induced MSC death. However, our findings indicate that once implanted, both PLGA/SIM and PLGA/MSC contributed to bone formation. We suggest that strategies to maintain the viability of MSCs after cultivation in PLGA/SIM will contribute to improvement of bone regeneration.

Indexed as

Bone RegenerationLactic AcidPolyglycolic AcidTissue ScaffoldsAnimalsCells, CulturedGlycolsMaleMesenchymal Stem CellsPolylactic Acid-Polyglycolic Acid CopolymerRatsRats, WistarSimvastatinTissue EngineeringGlycolsLactic AcidPolyglycolic AcidPolylactic Acid-Polyglycolic Acid CopolymerSimvastatin

Identifiers

PMID28291900
PMCPMC6379770
OpenAlexW2596343724

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.