ArticlePlant cell reports2017
Generation of stable nulliplex autopolyploid lines of Arabidopsis thaliana using CRISPR/Cas9 genome editing.
Article in Plant cell reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 33 citations in OpenAlex.
- Harnessing promoter elements to enhance gene editing in plants: perspectives and advances.Plant biotechnology journal · 2025Review
- Arabidopsis research in 2030: Translating the computable plant.The Plant journal : for cell and molecular biology · 2025Review
- Drought Stress Amelioration Attributes of Plant-Associated Microbiome on Agricultural Plants.Bioinformatics and biology insights · 2024Review
- Antioxidants of Non-Enzymatic Nature: Their Function in Higher Plant Cells and the Ways of Boosting Their Biosynthesis.Antioxidants (Basel, Switzerland) · 2023Review
- Targeted mutagenesis with sequence-specific nucleases for accelerated improvement of polyploid crops: Progress, challenges, and prospects.The plant genome · 2023Review
- Application of CRISPR/Cas9 Technology in Cancer Treatment: A Future Direction.Current oncology (Toronto, Ont.) · 2023Review
- The biological feasibility and social context of gene-edited, caffeine-free coffee.Food science and biotechnology · 2022Review
- Anthocyanin Biosynthesis Genes as Model Genes for Genome Editing in Plants.International journal of molecular sciences · 2021Review
- Gene dosage compensation of rRNA transcript levels in Arabidopsis thaliana lines with reduced ribosomal gene copy number.The Plant cell · 2021Article
- Barley's Second Spring as A Model Organism for Chloroplast Research.Plants (Basel, Switzerland) · 2020Review
- gRNA validation for wheat genome editing with the CRISPR-Cas9 system.BMC biotechnology · 2019Article
- Efficient Gene Transfer and Gene Editing in Sterlet (Frontiers in genetics · 2018Article
- CRISPR/Cascade 9-Mediated Genome Editing-Challenges and Opportunities.Frontiers in genetics · 2018Review
- CRISPR-based tools for plant genome engineering.Emerging topics in life sciences · 2017Article
- Assessing and Exploiting Functional Diversity in Germplasm Pools to Enhance Abiotic Stress Adaptation and Yield in Cereals and Food Legumes.Frontiers in plant science · 2017Review
- Plant genome editing with TALEN and CRISPR.Cell & bioscience · 2017Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA-guided endonuclease-mediated targeted mutagenesis using the clustered regularly interspersed short palindromic repeats (CRISPR)/Cas9 system has been successful at targeting specific loci for modification in plants. While polyploidy is an evolutionary mechanism enabling plant adaptation, the analysis of gene function in polyploid plants has been limited due to challenges associated with generating polyploid knockout mutants for all gene copies in polyploid plant lines. This study investigated whether CRISPR/Cas9 mediated targeted mutagenesis can generate nulliplex tetraploid mutant lines in Arabidopsis thaliana, while also comparing the relative efficiency of targeted mutagenesis in tetraploid (4x) versus diploid (2x) backgrounds. Using CRISPR/Cas9 genome editing to generate knockout alleles of the TTG1 gene, we demonstrate that homozygous nulliplex mutants can be directly generated in tetraploid Arabidopsis thaliana plants. CRISPR/Cas9 genome editing now provides a route to more efficient generation of polyploid mutants for improving understanding of genome dosage effects in plants.
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Registered trials
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