Dielectrophoresis study of temporal change in internal conductivity of single CHO cells after electroporation by pulsed electric fields.

E Salimi, K Braasch, M Butler, D J Thomson, G E Bridges

Abstract read

In one paragraph

Article in Biomicrofluidics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it

0cells of the map it votes in

3citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

Recent Approaches to Design and Analysis of Electrical Impedance Systems for Single Cells Using Machine Learning.Sensors (Basel, Switzerland) · 2023
Review
Recent Advances in Microscale Electroporation.Chemical reviews · 2022
Review
Simultaneous electroporation and dielectrophoresis in non-electrolytic micro/nano-electroporation.Scientific reports · 2018
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

E SalimiDepartment of Electrical and Computer Engineering, University of Manitoba , Winnipeg, Manitoba R3T 5V6, Canada.

K BraaschDepartment of Microbiology, University of Manitoba , Winnipeg, Manitoba R3T 2N2, Canada.

M ButlerDepartment of Microbiology, University of Manitoba , Winnipeg, Manitoba R3T 2N2, Canada.

D J ThomsonDepartment of Electrical and Computer Engineering, University of Manitoba , Winnipeg, Manitoba R3T 5V6, Canada.

G E BridgesDepartment of Electrical and Computer Engineering, University of Manitoba , Winnipeg, Manitoba R3T 5V6, Canada.ORCID 0000-0001-8147-9882

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Applying sufficiently strong pulsed electric fields to a cell can permeabilize the membrane and subsequently affect its dielectric properties. In this study, we employ a microfluidic dielectrophoresis cytometry technique to simultaneously electroporate and measure the time-dependent dielectric response of single Chinese hamster ovary cells. Using experimental measurements along with numerical simulations, we present quantitative results for the changes in the cytoplasm conductivity of single cells within seconds after exposure to 100

Identifiers

PMID28289483

DOI10.1063/1.4975978

PMCPMC5315669

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.