ArticleAnnals of burns and fire disasters2016
Fast and specific detection of Pseudomonas Aeruginosa from other pseudomonas species by PCR.
Article in Annals of burns and fire disasters, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Emerging XDR and MDR Pseudomonas aeruginosa strains from Oreochromis niloticus reveal high pathogenicity, virulence determinants, antimicrobial resistance genes, and oprL sequence diversity.Scientific reports · 2026Article
- Test Strips Based on Gated Nanoporous Anodic Alumina for the Rapid and Accurate Detection ofAnalytical chemistry · 2026Article
- Profile of predominant gram-negative pathogenic bacteria in River Sosiani and wastewater systems in Eldoret Town, Uasin Gishu County, Kenya.Microbiology spectrum · 2025Article
- Accurate Diagnosis ofAntibiotics (Basel, Switzerland) · 2025Article
- Miniaturized Devices for Isothermal Amplification and Photometric Quantification of Pseudomonas Aeruginosa.IEEE open journal of engineering in medicine and biology · 2025Article
- Article
- The impact of quorum sensing and biofilm formation on antimicrobial resistance and virulence of XDR and MDRIranian journal of veterinary research · 2024Article
- A novel, low-cost microfluidic device with an integrated filter for rapid, ultrasensitive, and high-throughput bioburden detection.Scientific reports · 2023Article
- Prevalence and molecular analysis of antibiotic resistance ofIranian journal of microbiology · 2023Article
- Direct multiplex recombinase polymerase amplification for rapid detection ofMolecular biology research communications · 2022Article
- Article
- Review
- Simple, Rapid and Sensitive Detection of Pseudomonas aeruginesa by Colorimetric Multiple Cross Displacement Amplification.Current medical science · 2020Article
- Article
- Establishment of loop-mediated isothermal amplification for rapid detection ofExperimental and therapeutic medicine · 2019Article
- Rapid Calorimetric Detection of Bacterial Contamination: Influence of the Cultivation Technique.Frontiers in microbiology · 2019Article
- Detection of Overexpression of Efflux Pump Expression in Fluoroquinolone-ResistantInternational journal of applied & basic medical researchArticle
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pseudomonas aeruginosa is an important life-threatening nosocomial pathogen that plays a prominent role in wound infections of burned patients. We designed this study to identify the isolates of P. aeruginosa recovered from burned patients at the genus and species level through primers targeting oprI and oprL genes, and analyzed their antimicrobial resistance pattern. Over a 2-month period, wound samples were taken from burned patients and plated on MacConkey agar. All suspected colonies were primarily screened for P. aeruginosa by a combination of phenotypic tests. Molecular identifications of colonies were done using specific primers for oprI and oprL genes. Bacterial isolates were recovered from burn wound infections. Based on phenotypical identification tests, 138 (34%) P. aeruginosa isolates were identified; whereas by molecular techniques, just 128 P. aeruginosa yielded amplicon of oprL gene using species-specific primers, verifying the identity of P. aeruginosa; the others yielded amplicon of oprI gene using genus-specific primers, confirming the identity of fluorescent pseudomonads. This study indicates that molecular detection of P. aeruginosa in burn patients employing the OprL gene target is a useful technique for the early and precise detection of P. aeruginosa. PCR detection should be carried out as well as phenotypic testing for the best aggressive antibiotic treatment of P. aeruginosa strains at an earlier stage. It also has significant benefits on clinical outcomes.
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Registered trials
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