ArticleJournal of biotechnology2017
A comparative approach to recombinantly produce the plant enzyme horseradish peroxidase in Escherichia coli.
Article in Journal of biotechnology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 48 citations in OpenAlex.
- A Simple and Cost-Efficient Method for the Production of Recombinant Horseradish Peroxidase in E. coli.Molecular biotechnology · 2025Article
- Crystal structure of ferric recombinant horseradish peroxidase.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2025Article
- Production of high-affinity glycosylated anti-mouse conjugated nanobodies inFrontiers in bioengineering and biotechnology · 2025Article
- Glycosylation increases active site rigidity leading to improved enzyme stability and turnover.The FEBS journal · 2023Article
- Antibody-Invertase Fusion Protein Enables Quantitative Detection of SARS-CoV-2 Antibodies Using Widely Available Glucometers.Journal of the American Chemical Society · 2022Article
- Expression of full and fragment-B of diphtheria toxin genes inClinical and experimental vaccine research · 2022Article
- Conversion of lignin-derived 3-methoxycatechol to the natural product purpurogallin using bacterial P450 GcoAB and laccase CueO.Applied microbiology and biotechnology · 2022Article
- Recombinant cellobiohydrolase of Myceliophthora thermophila: characterization and applicability in cellulose saccharification.AMB Express · 2021Article
- Enzyme prodrug therapy: cytotoxic potential of paracetamol turnover with recombinant horseradish peroxidase.Monatshefte fur chemie · 2021Article
- Construction of a new T7 promoter compatible Escherichia coli Nissle 1917 strain for recombinant production of heme-dependent proteins.Microbial cell factories · 2020Article
- Scalable High-Performance Production of Recombinant Horseradish Peroxidase fromInternational journal of molecular sciences · 2020Article
- Translocation from the chloroplast stroma into the thylakoid lumen allows expression of recombinant epidermal growth factor in transplastomic tobacco plants.Transgenic research · 2020Article
- Stepwise optimization of recombinant protein production in Escherichia coli utilizing computational and experimental approaches.Applied microbiology and biotechnology · 2020Review
- Article
- Improving the Performance of Horseradish Peroxidase by Site-Directed Mutagenesis.International journal of molecular sciences · 2019Article
- Perspectives of inclusion bodies for bio-based products: curse or blessing?Applied microbiology and biotechnology · 2019Review
- Heterologous Expression, Purification and Characterization of a Peroxidase Isolated from Lepidium draba.The protein journal · 2017Article
- Thermostable exoshells fold and stabilize recombinant proteins.Nature communications · 2017Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Horseradish peroxidase (HRP) is used in various biotechnological and medical applications. Since its isolation from plant provides several disadvantages, the bacterium Escherichia coli was tested as recombinant expression host in former studies. However, neither production from refolded inclusion bodies nor active enzyme expression in the periplasm exceeded final titres of 10mg per litre cultivation broth. Thus, the traditional way of production of HRP from plant still prevails. In this study, we revisited the recombinant production of HRP in E. coli and investigated and compared both strategies, (a) the production of HRP as inclusion bodies (IBs) and subsequent refolding and (b) the production of active HRP in the periplasm. In fact, we were able to produce HRP in E. coli either way. We obtained a refolding yield of 10% from IBs giving a final titre of 100mgL
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.